GC-MS法同时检测南瓜果实肌醇类和单、双糖类物质的研究

Simultaneously quantifying inositols and mono-, di-saccharides in pumpkin fruit by GC-MS method

为了高效同时测定南瓜果实中的肌醇类和单、双糖类物质,采用气相色谱-质谱(Gas chromatography mass spectrometer,GC-MS)联用技术,以不同提取试剂、不同提取温度和步骤的不同来确定南瓜肌醇的最佳提取方法,并采用对南瓜果实样品进行全谱扫描和选择离子扫描。得到最优方案:南瓜样品在60%甲醇溶液于70℃水浴1 h,真空干燥以后,加水重新溶解后直接进行衍生化,其提取效果最佳;采用GC-MS扫描确定了南瓜果实样品中的主要代谢成分半乳糖、葡萄糖、D-手性肌醇、肌-肌醇、果糖和蔗糖。试验方法简单高效,GC-MS的进样时间短,出峰完全,可同时分析多个物质,重复性好,结果精确稳定。

To simultaneously measure substance of various inositol and mono-, di-saccharides in pumpkin, gas chromatography mass spectrometer（GC-MS） was used for full spectrum scanning and selective ion scanning for pumpkin fruit samples with different extracting reagents and temperatures and modifying re-dissolving steps to explore optimum extract method for inositols and sugars in pumpkin. The optimal method is pumpkin fruit samples were extracted by 60% methanol at 70 ℃ water bathing for 1 h; after vacuum drying, re-dissolving in water, samples were finally derivatized, its extraction effects was the best. It was identified by this GC-MS scanning method, the main metabolite components of pumpkin fruit samples were galactose, glucose, DCI, MI, fructose, sucrose. The experiment method was simple and efficient, loaded the samples time was short, the peak was completely. The antidiabetic metabolites, inositols and sugars could be quantified simultaneously with excellent repetition, and the results were accurate and steady.