赤芝水提取物中的三萜类成分

Ganoderma triterpenoids from aqueous extract of Ganoderma lucidum

采用硅胶、ODS、Sephadex LH-20、HPLC等色谱技术手段,从赤芝水提物中分离得到1个新的灵芝三萜酸和18个已知类似物。利用理化性质和波谱数据,鉴定新化合物为2β-acetoxy-3β,25-dihydroxy-7,11,15-trioxo-lanost-8-en-26-oic acid(1)。通过与文献数据比较,已知化合物分别鉴定为ganoderic acid H(2),12β-acetoxy-3β,7β-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid(3),ganoderenic acid D(4),ganoderic acid C1(5),ganoderic acid G(6),3β,7β-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid(7),ganoderic acid B(8),ganoderic acid C6(9),3β,15α-dihydroxy-7,11,23-trioxo-lanost-8,16-dien-26-oic acid(10),ganoderic acid A(11),ganolucidic acid A(12),lucidenic acid E2(13),lucidenic acid N(14),lucidenic acid P(15),lucidenic acid B(16),lucidenic acid A(17),lucidenic acid C(18),lucidenic acid L(19)。虽然化合物2~19在赤芝中已经报道,但是化合物1是新化合物。因此,该研究进一步丰富了赤芝的化学成分,尤其是填补了赤芝传统水煎剂化学成分研究的空白。

A new triterpenoid and 18 analogues were isolated from the water extract of Ganoderma lucidum by column chromatographic techniques, including silica gel, ODS, Sephadex LH-20, and HPLC. The new compound was elucidated as 2β-acetoxy-3β,25-dihydroxy-7,11,15-trioxo-lanost-8-en-26-oic acid on the basis of analyses of extensive spectroscopic data and its physicochemical properties. Comparison of NMR data with those reported in literature, the known analogues were determined as ganoderic acid H （2）, 12β-acetoxy-3β,7β-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid （3）, ganoderenic acid D （4）,ganoderic acid C1 （5）,ganoderic acid G （6）,3β,7β-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid （7）,ganoderic acid B （8）,ganoderic acid C6 （9）,3β,15α-dihydroxy-7,11,23-trioxo-lanost-8,16-dien-26-oic acid （10）,ganoderic acid A （11）,ganolucidic acid A （12）,lucidenic acid E2 （13）,lucidenic acid N （14）,lucidenic acid P （15）, lucidenic acid B （16）,lucidenic acid A （17）,lucidenic acid C （18）,and lucidenic acid L （19）, respectively. Compound 1 is new compound and compounds 2-19 have been reported from G. lucidum. The present study enriches the knowledge of the chemical constituent of G. lucidum and completes chemical investigation of water decoction that is traditional use of G. lucidum.