豆叶九里香的化学成分研究

Chemical constituents from Murraya euchrestifolia

利用开放的硅胶、ODS和Sephadex LH-20柱色谱及半制备型高效液相等色谱方法对豆叶九里香Murraya euchrestifolia中的化学成分进行分离纯化,结合理化性质和MS,NMR等波谱学数据对化学结构进行解析。结果从豆叶九里香95%乙醇提取物的二氯甲烷部位共分离并鉴定了18个化合物,分别为sakuranetin(1),eriodictyol-7,4'-dimethyl ether(2),isosakuranetin(3),5-hydroxy-7,4'-dimethoxyflavanone(4),eriodictyol-7-methyl ether(5),lichexanthon(6),5,6,7-trimethoxycoumarin(7),5-hydroxy-6,8-dimethoxycoumarin(8),8-hydroxy-6-methoxy-3-n-pentylisocoumarin(9),ethyl caffeate(10),4-hydroxy-3,5-dimethoxycinnamic acid ethyl ester(11),methyl 3-(5'-hydroxyprenyl)-coumarate(12),(E)-coniferol(13),β-hydroxypropiovanillone(14),3-hydroxy-7,8-didehydro-β-ionone(15),3β-hydroxy-5α,6α-epoxy-7-megastigmen-9-one(16),grasshopper ketone(17),4-hydroxy-3,5-dimethoxybenzaldehyde(18),其中化合物1~15和18为首次从九里香属植物中分离得到,16和17为首次从该植物中分离得到。

The open silica gel, ODS, and Sephadex LH-20 column chromatography, along with the semi-preparative HPLC was used to isolate and purify the chemical constituents from Murraya euchrestifolia. The structures of the isolates were elucidated by their physiochemical properties, NMR, and MS spectroscopic data, as well as comparison with literature data. Eighteen compounds were isolated from the CH2Cl2 fraction of the 95% aqueous EtOH extract of M. euchrestifolia, and their structures were identified as sakuranetin （1）, eriodictyol-7,4＇-dimethyl ether （2）, isosakuranetin （3）, 5-hydroxy-7,4＇-dimethoxyflavanone （4）, eriodictyol-7-methyl ether （5）, lichexanthon （6）, 5,6,7-trimethoxycoumarin （7）, 5-hydroxy-6,8-dimethoxycoumarin （8）, 8-hydroxy-6-methoxy-3-n-pentylisocoumarin （9）, ethyl caffeate （10）, 4-hydroxy-3,5-dimethoxycinnamic acid ethyl ester （11）, methyl 3-（5＇-hydroxyprenyl）-coumarate （12）, （E）-coniferol （13）, β-hydroxypropiovanillone （14）, 3-hydroxy-7,8-didehydro-β-ionone （15）, 3β-hydroxy-5α, 6α-epoxy-7-megastigmen-9-one （16）, grasshopper ketone （17）, and 4-hydroxy-3,5-dimethoxybenzaldehyde （18）. Compounds 1-15 and 18 were first obtained from the plants of Murraya genus, and compounds 16 and 17 were isolated from M. euchrestifolia for the first time.