5种石斛对四氯化碳致小鼠急性肝损伤的保护作用

Protective effects of five different types of Dendrobium on CCl_4-induced liver injury in mice

该文旨在研究比较霍山石斛、铁皮石斛(霍山)、铁皮石斛(云南)、铜皮石斛和河南石斛等5种石斛对四氯化碳急性肝损伤的保护作用。实验将C57BL/6小鼠随机分为空白对照组、模型对照组、5种石斛高(7.5 g·kg^(-1))和低(1.25 g·kg^(-1))2个剂量组。各组连续给药2周,末次给药2 h后,除对照组腹腔注射橄榄油外,其余各组腹腔注射0.5%CCl4橄榄油溶液。16 h后,采集小鼠血清及肝组织,测定小鼠血清中ALT,AST的活性;并检测肝组织中SOD和MDA的水平;计算肝脏指数并观察肝组织病理学变化,荧光定量PCR技术检测肝组织中IL-1β,IL-6和TNF-α的mRNA的表达水平。结果显示,与模型组相比,各石斛组均能降低小鼠血清指标ALT和AST水平,使肝组织中MDA含量下降,SOD活性升高,减轻CCl4对肝脏的病理损伤。此外,霍山石斛肝组织中IL-1β,IL-6和TNF-α的mRNA表达水平显著下调。结果表明,5种石斛对四氯化碳急性肝损伤具有保护作用。但不同产地石斛和不同种石斛的保肝效果有所不同,其中霍山石斛效果最为显著,其他几种石斛保肝效果依次为铁皮石斛(云南)、铁皮石斛(霍山)、河南石斛和铜皮石斛,这可能与不同基源品种石斛化学成分不同有关。

Abstract：This study aims to investigate the protective effect of Dendrobium huoshanense, D.officinale（Huoshan）, D.officinale（Yunnan）, D.moniliforme and D. henanense on CCl4-induced hepatic damage in mice. C57BL/6 mice were randomly divided into control group, model group, high-dose（7.5 g·kg^-1） and low-dose （1.25 g·kg^-1） groups of the five Dendrobium. Each group was intragastrically administered with drugs for 2 weeks. The control group was intraperitoneally injected with Olive oil solution, while the other groups were intraperitoneally given 0.5%CCl4combined with Olive oil solution 2 h later after the last administration. Subsequently, ALT and AST activities in serum, SOD activities and MDA contents in liver tissues were determined in all groups 16 h later after administration. The liver index was calculated, and hepatic histopathological examination was performed. The mRNA expressions of IL-1β, IL-6 and TNF-α were analyzed by Real-time PCR. Compared with the CCl4 model group, the activities of ALT and AST in serum decreased significantly in the five different Dendrobium groups. Meanwhile, in liver tissues, the levels of MDA reduced obviously, while the SOD activities markedly increased. Furthermore, liver tissue damage induced by CCl4 was ameliorated according to the histopathological examination. IL-1β, IL-6 and TNF-α mRNA expressions in D.huoshanense-treated liver tissues were significantly decreased. In conclusion, the five different Dendrobium groups showed hepatoprotective effects on CCl4-induced acute liver injury in mice. However, there were differences among Dendrobium of different types and origins. The protect effect of D.huoshanense is the most obvious, and the order of the protective effect of the other Dendrobium from high to low is D.officinale（Yunnan）, D. officinale（Huoshan）, D.henanense and D.moniliforme. The differences between the different types of Dendrobium might be related to their chemical components.