正交试验优选养血接骨胶囊的水提工艺

Optimization of Water Extraction Technology of Yangxue Jiegu Capsule by Orthogonal Test

目的:优选养血接骨胶囊水提工艺。方法:以加水量、煎煮时间、煎煮次数为考察因素,以阿魏酸含量、淫羊藿苷含量和得膏率的综合评分为考察指标,采用正交试验优选养血接骨胶囊的最佳水提工艺并进行验证试验。结果:养血接骨胶囊最优提取工艺为加10倍量水提取3次,每次1 h;验证试验中阿魏酸、淫羊藿苷含量分别为0.38、1.23 mg/g(n=3),得膏率为28.2%(n=3),综合评分为97.11(RSD=2.77%,n=3)。结论:优选的提取工艺简便可行、方法准确可靠,可用于养血接骨胶囊的提取制备。

OBJECTIVE： To optimize the water extraction technology of Yangxue Jiegu capsule. METHODS： Using adding amount of water, decoction time and decoction times as investigation factors, ferulic acid content, icariin content and yield rate as investigation indexes, orthogonal test was used to optimize the optimal water extraction technology of Yangxue Jiegu capsule; and verification test was conducted. RESULTS： The optimized extraction technology was 10-fold water, extracting 3 times, 1 h once. Contents of ferulic acid and icariin were 0.38, 1.23 mg/g （n=3）, yield rate was 28.2% （n=3）, average comprehensive score was 97.11 （RSD=2.77%, n=3）. CONCLUSIONS： Optimized extraction technology is feasible, accurate, reliable, and can be used for the extraction and preparation of Yangxue Jiegu capsule.