溴氰菊酯对真鲷肝胰脏组织及细胞DNA的损伤

Damage of deltamethrin on tissue and cell DNA in hepatopancreas of Pagrosomus major

为研究溴氰菊酯亚急性染毒对真鲷肝胰脏的毒性作用,将真鲷分为5组进行不同剂量半静置染毒.染毒25 d后取真鲷肝胰脏进行组织切片显微观察,并采用彗星试验技术对其肝胰脏细胞进行DNA损伤分析.结果表明:在0.025、0.125、0.250和0.375μg·L^(-1)暴露浓度下,真鲷肝胰脏组织出现了不同程度的淤血、细胞核浓缩、细胞坏死等病理性损伤,且染毒浓度越高组织细胞损伤越显著.与空白对照组相比,各染毒浓度组肝胰脏细胞DNA均有不同程度损伤,彗星拖尾率、彗尾DNA相对含量、Olive距等指标均与对照有显著性差异.一元回归分析表明,染毒浓度与拖尾率、彗星尾长等参数呈极显著正相关;染毒浓度与各指标均呈线性关系,回归方程拟合度(R)极高,范围为0.909~0.996.表明溴氰菊酯对真鲷肝胰脏组织和细胞DNA均可产生不同程度损伤,且损伤程度与染毒浓度之间具有高度线性相关关系.

In order to study the toxic effect of deltamethrin on the hepatopancreas of Pagrosomus major with sub-acute exposure, Pagrosomus major were divided into 5 groups with different doses of semi-static exposure. The damage of the hepatopancreas tissue of Pagrosomus major was analyzed by microscopy observation and the DNA damage of the cells was analyzed by comet assay technique af- ter 25 days exposure. The results showed that different degrees of congestion, nuclear enrichment and cell necrosis appeared in hepatopancreas of 0.025, 0.125, 0.250, 0.375 μg· L-l exposure concentrations. The higher the exposure concentration, the variations of tissue and cell were more significant. DNA damages in the hepatopancreas cells of all exposed group were observed. The co- met rate, percentage of tail DNA and the olive moment were significantly different from the control group （P〈0.05）. Linear regression analysis showed that the exposure concentration was significant- ly positively correlated with the tailing rate and the tail length （P〈0.01）. The indexes were signifi- cantly related to the concentration of deltamethrin exposure with the coefficient of determination be- tween 0.909-0.996. It was suggested that deltamethrin could cause various degree of damage to the tissue and cellular DNAs in the hepatopanereas of Pagrosomus major in a linear responsive manner.