紫花苞舌兰类原球茎诱导及植株再生

Inducement of Philippine Ground Orchid(Spathoglottis plicata) ProtocormLike Bodies and Plant Regeneration

为建立紫花苞舌兰类原球茎离体再生体系,本研究以其腋芽为外植体,研究了不同激素浓度配比、添加剂对其类原球茎诱导、分化及生根诱导的影响。结果表明,外植体在1/2 MS+6-BA2.0 mg/L+NAA0.2 mg/L+AC(活性炭)0.2 g/L+CW(椰汁)10%培养基上培养35 d后,获得无菌苗,其成活率达80%以上;无菌苗的顶端分生组织在1/2 MS+10 mg/L Picloram培养基上的类原球茎的诱导率最高,达75%;类原球茎在1/2 MS+6-BA 2.0 mg/L+IBA 0.2 mg/L培养基上培养45 d后分化出小苗,分化率为54%;小苗在1/2 MS+6-BA 0.5 mg/L+IBA 1.0 mg/L+AC 0.2 g/L培养基上生根培养35 d后,生根率达85%以上;小苗驯化移栽成活率达95%,长势良好。

In order to establish a regeneration system in Spathoglottis plicata, its axillary bud was used as explant to study the effect of different hormone concentration and additives on PLBs induction, differentiation and rooting. The results showed that sterile plantlets were obtained under the condition of 1/2 MS＋2.0 mg/L 6-BA＋0.2 mg/L NAA＋0.2 g/L AC （activated carbon）＋ 10%, CW （coconut juice） after 35 days and its survival rate was higher that 80%. The PLBs induction of apical meristem reached its top under the condition of 10 mg/L Picloram＋l/2 MS, and the highest induction rate of PLBs clusters was 75%. Plantlets were differentiated from the PLBs under the condition of 1/2 MS＋2.0 mg/L 6-BA＋0.2 mg/L IBA after 45 d and the differentiation rate was 54%. The regeneration rate could reach up to 85% under the condition of 1.0 mg/L IBA＋0.5 mg/L 6-BA＋0.2 g/L AC after 35 days. The survival rate of transplanted seedlings reached 95% with excellent growth.