云南汉族人群NKG2D基因多态性与非小细胞肺癌发生和发展的相关性

The Correlation of NKG2D Gene Polymorphisms with the Initiation and Development of Non-small-cell Lung Cancer in A Yunnan Han Population

目的:探讨云南汉族人群活化性受体NKG2D基因单核苷酸多态性(SNP)与非小细胞肺癌的相关性。方法:选择云南汉族非小细胞肺癌患者467例(肺癌Ⅰ+Ⅱ期组151例,肺癌Ⅲ+Ⅳ期组316例)和健康对照384例(对照组),采用Taq Man探针基因分型方法对NKG2D基因的2个SNPs(rs2255336和rs2617160)位点进行基因分型,研究其等位基因、基因型及所构建的单倍型在肺癌Ⅰ+Ⅱ期组、Ⅲ+Ⅳ期及对照组人群中的分布差异。结果:rs2255336和rs2617160位点的等位基因及基因型在肺癌Ⅰ+Ⅱ期组、肺癌Ⅲ+Ⅳ期组和对照组中的分布频率的差异无统计学意义(P>0.05);遗传模式分析显示,rs2255336和rs2617160在Ⅰ+Ⅱ期肺癌组和Ⅲ+Ⅳ期肺癌组最优的遗传模式均为Recessive模式,其中CC-CT基因型相对于TT基因型可能是肺癌发展的保护性因素(P=0.040,OR=0.26,95%CI:0.06-1.17);rs2617160位点TT-AT基因型相对于AA基因型可能是肺癌发展的保护性因素(P=0.045,OR=0.60,95%CI:0.36-1.00)。结论:NKG2D基因rs2255336和rs2617160 SNPs位点可能与云南汉族人群非小细胞肺癌的发展有关。

Objective： To investigate the correlation of the singe nucleotide polymorphisms （SNP） of natural-killer group 2, member D （NKG2D） gene with non-small cell lung cancer （NSCLC） in a Yunnan Han population. Method： TaqMan asssy was used to study genotype 2 SNPs （rs2255336 rs2617160） in NKG2D gene in 467 NSCLC patients （including patients in I ＋ Ⅱ and Ⅲ＋Ⅳ stages, namely group I ＋ Ⅱ and group m ＋ IV ） and 490 healthy people （control group）. The distribution of alleles, genotypes and the constructed haplotypes were analyzed. Results： The allelic and genotypicfrequencies of the 2 SNPs were not significantly different between I ＋ Ⅱ, and control group, and be- tween Ⅲ＋Ⅳ and control group （ P 〉 0.05 ）. While results of inheritance mode analysis showed that the best fit inheritance model for rs2255336 and rs2617160 in 2 lung cancer groups was recessive mod- el. The CC-CT genotype of rs2255336 （ P = 0. 040, OR = 0. 26, 95 % CI： 0. 06 - 1.17 ） and the Ⅲ＋Ⅳ genotype of rs2617160 （P =0. 045 ,OR =0.60, 95% CI：O. 36 -1.00） might be the protective factors against NSCLC development. Conclusions： Polymorphisms （ rs2255336 and rs2617160） in NKG2D gene might correlate with NSCLC in this Yunnan Han population.