Micro-PET在大鼠体内^(18)F-FDG药物浓度监测中的应用

Evaluation of the Application Value of Micro-PET in Monitoring the Concentration of ^(18)F-FDG in vivo

目的:探讨微型正电子发射断层扫描(Micro-PET)在大鼠体内氟代脱氧葡萄糖(^(18)F-FDG)药物浓度监测中的价值。方法:采用自身前后对照法将10只大鼠分别作为实验组和对照组,按注射^(18)F-FDG药物后的时间分为实验早期(60 min以内)和实验后期(60 min以后);实验组大鼠注射^(18)F-FDG后,运用Micro-PET进行实验早期连续动态显像和实验后期不同时间点静态显像,显像完毕后以左心室腔为感兴趣区(ROI)测定大鼠血药浓度;10 d后给予自身对照组同样剂量^(18)F-FDG,在对应时间点以断尾采血方式获得血样标本,通过γ计数仪测定血药浓度;对实验组及对照组相应时间点的药物浓度采用配对样本t检验,对实验早期两组数据进行Pearson相关性分析和组内一致性检验。结果:实验早期两组血药浓度比较,差异无统计学意义(P>0.05),Pearson相关系数r为0.986(P=0.000),组内相关系数ICC为0.976(95%CI:0.891~0.995),两组数据具有较好的相关性及一致性;实验后期两组血药浓度比较,差异有统计学意义(P<0.05)。结论:Micro-PET可连续动态监测给药后大鼠体内早期^(18)F-FDG药物浓度的变化,可作为活体条件下测定大鼠体内^(18)F-FDG浓度的有效方法。

Objective： To investigate the value of Micro-PET in monitoring the concentration of flu- orodeoxyglucose （18F-FDG） in rats. Methods： 10 rats were divided into experimental group and con- trol group by the self-contrast method, and the experiment stage were divided into early stage （within 60 min） and later stage （after 60 min） according to the time after injecting of 18F-FDG. After injection of 18F_FDG to the experimental group of rats, micro-PET was adopted to conduct continuous dynamic imaging at the early stage and static imaging at different time points at the later stage. After imaging, plasma 18F-FDG concentration of rats was determined in the left ventrieular cavity （ROI）. 10 days lat- er, the same dose of is F-FDG was injected into control group, blood samples was obtained by the tail blood method at the corresponding time point, and blood 18F-FDG concentration was measured by gam- ma counter; The paired sample t test was used to test drug concentration at the corresponding time point in experimental group and the control group, and the experimental data of early stage of the two groups were analyzed by Pearson correlation and consistency test. Results： There was no statisticallysignificant difference in blood is F-FDG drug concentrations in early stage between the two groups （P 〉 O. 05 ）. Pearson correlation coefficient r was 0. 986 （P = O. 000）, intraclass correlation coefficient ICC was 0. 976 （95% CI： O. 891 -0. 995 ）, and the data of the two groups have good correlation and con- sistency. There was a significant difference in blood 18F-FDG drug concentration between the two groups at the later stage of the experiment （ P 〈 0, 05 ）. Conclusion： Micro-PET can continuously and dynamically monitor the change of drug concentration of JSF-FI）G in rats at the early stage, and can be used as an effective method of determination of concentration of lSF-FDG in vivo.