类雪旺细胞与可吸收明胶海绵体外混合培养的实验研究

Coculture of Schwann-like cells and absorbable gelatin sponge in vitro

目的探讨可吸收明胶海绵作为类雪旺细胞组织工程载体的可能性，为体内移植治疗周围神经损伤奠定基础。方法体外培养骨髓间充质干细胞（bone mesenchymal stem cells，BMSCs），并诱导为类雪旺细胞，免疫荧光检测细胞标志物，将诱导后的类雪旺细胞接种于可吸收明胶海绵，通过MTF实验检测接种后细胞的活性，扫描电镜观察细胞的形态变化、黏附、增殖等隋况。结果骨髓间充质干细胞经诱导分化后，细胞呈典型雪旺细胞的形态，免疫荧光检测S-100、GFAP表达阳性，并与明胶海绵混合培养后，可黏附于可吸收明胶海绵表面，并正常增殖。结论可吸收明胶海绵具有良好的生物相容性，可作为组织工程载体搭载类雪旺细胞。

Objective To explore the possibility of using absorbable gelatin sponge as Schwann- like cell matrix for in vivo transplantation. Methods Bone mesenchymal stem cells （BMSCs） were cultured and expanded in LG-DMEM with 10% fetal bovine serum. The passage 3 BMSCs were induced to differentiate into Schwann-like cells and then seeded onto absorbable gelatin sponge. The adhesion and proliferation of Schwann-like cells on absorbable gelatin sponge were determined by MTT test and scanning electron microscope. Results The flow cytometry showed that BMSCs had positive response for CD90 and CD29. Immunofluorescence showed that the induced Schwann-like cells had positive response for S-100 and GFAP. These cells were co-cultured with absorbable gelatin sponge and demonstrated that Schwann-like cells attached to the surface of the gelatin, proliferated and secreted extracellular matrix. Conclusion Schwann-like cells are able to attach and proliferate on the absorbable gelatin sponge. The absorbable gelatin sponge can be used as a tissue engineering carrier.