树鼩帕金森病(PD)模型的建立及对其的行为学和形态学研究

Establishment of Parkinson's disease model of tree shrew and research in behavior and morphology of PD

目的:建立树鼩的帕金森病(PD)模型,用行为学和形态学相结合的方法对模型进行验证。方法:应用三种方法制备树鼩PD模型,通过行为学和形态学相结合的方法对模型予以验证。结果:MPTP组树鼩的旱迷宫全天总反应时间、错误反应次数分别比6-OHDA组和罗滕酮组显著增高(P<0.05);MPTP组树鼩的爬杆实验所用时间分别较6-OHDA组和罗滕酮组显著增长(P<0.05)。与树鼩中脑腹侧的对照侧相比,6-OHDA组注射侧和罗滕酮组注射侧中脑腹侧的酪氨酸羟化酶(TH)阳性神经细胞数显著减少(P<0.01);而MPTP组双侧中脑腹侧的TH阳性细胞数均显著减少(对照侧为P<0.01,注射侧为P<0.05)。结论:腹腔注射MPTP制作的树鼩PD模型为较理想的模型,值得在神经科学研究中推广应用。

Objective： To establish the Parkinson＇s disease （PD） model of tree shrew, and to verify it by behavior and morphological detection. Methods： Three methods were used to set up PD tree shrew model. Behavioral method and mor- phological method were combined together to verify the success of PD tree shrew model. Results： The total response time and error reaction times of drought maze detection in MPTP injection group was significantly longer than that of 6-OHDA and Luotengtong injection group, respectively （ P 〈 0.05 ）. As for climbing pole experiment, the time consuming in MPTP group was markedly longer than that of 6-OHDA and Luotengtong injection group respectively （P 〈 0.05）. The number of TH pos- itive cells in the injected side of ventral mid-brain in 6-OHDA injected group and Luotengtong injected group was markedly reduced when compared with the normal control side （P 〈0.01 ）. Notably, in the MPTP injected group, TH positive cells significantly decreased in both injected and normal control side （P 〈 0.05, P 〈 0.01, respectively）. Conclusion： The tree shrew PD model established by MPTP intraperitoneal injection has been considered as a relatively more ideal one among the three methods we used, therefore deserved to be widely applied in neuroscience research.