贵州白山羊L-FABP基因遗传变异对生长性状的影响

Influence of Genetic Variation of L-FABP Gene on the Growth Traits of Guizhou White Goat

本研究旨在探讨肝脏型脂肪酸结合蛋白(L-FABP)基因遗传变异与贵州白山羊生长性状的关联性,以期得到能够影响贵州白山羊各群体生长性状显著效应的分子遗传标记。试验采用DNA池、PCR-SSCP法结合PCR产物直接测序对贵州白山羊L-FABP基因4个外显子进行SNPs筛选,并利用SPSS 18.0统计软件分析H-FABP基因遗传变异与生长性状间的相关性。多态位点检测表明,贵州白山羊L-FABP基因内含子两处存在C4557T和A4575G两个多态基因座;遗传效应分析表明,L-FABP基因C4557T和A4575G位点多态座位有利于提高贵州白山羊体重、体高、体斜长和管围,其中基因型CCGG有利于提高贵州白山羊成年母羊的体高和体斜长;TTGG有利于提高贵州白山羊周岁母羊的体重和管围。研究结果初步推测L-FABP基因可能影响贵州白山羊部分生长性状的主效基因或与主效基因连锁,其多态座位可能作为山羊生长性状选择的有效遗传标记。

The aim of this study was to explore the correlation between the genetic variation of L - FABP gene and the growth traits of Guizhou white goat in order to obtain molecular genetic markers that could affect the goat＇ s growth traits. The SNPs of four different exons of L - FABP genes of Guizhou white goat were screened by DNA pooling and PCR - SSCP combined with PCR product direct sequencing. The correlation between the genetic variation of L - FABP gene and the growth traits was analyzed by SPSS （ 18.0 ） software. Examination of the polymorphism loci showed that two SNPs （ C4557T and A4575G） were presented in the Intron 2 of L - FABP gene. The effect analysis showed that the polymorphic loci of C4557T and A4575G sites of L - FABP gene can improve body weight, body height, body length and pipe circumference. The genotype CCGG can be in favour of improving body height and body length of adult ewes. The genotype TTGG can be in favour of improving body weight and pipe circumference in aging ewes. The results indicated that L - FABP gene wouldbe a potential major gene or a gene linked to other major genes affecting goat growth traits ; its polymorphic lo- ci could be used as effective genetic markers for goat growth traits to guide the molecular breeding of goats.