中国汉族老年人糖尿病与瘦素受体基因rs1137100和rs1137101多态性位点相关性

Relationship of diabetes mellitus in older Han adults in China with leptin receptor gene rs1137100 and rs1137101 polymorphrism

目的探讨汉族老年人糖尿病与瘦素受体基因rs1137100和rs1137101多态性位点(SNP)的相关性。方法采用Taqman方法检测241例汉族老年人糖尿病患者和270例汉族健康老年人的瘦素受体基因rs1137100和rs1137101基因型及等位基因频率分布状况,分析各SNP位点基因型与糖尿病的相关性,同时测定所有研究对象的血脂、血糖、血浆瘦素和胰岛素水平。结果 rs1137100位点GG、GA和AA基因型在糖尿病组和正常对照组之间的分布频率分别为73.0%、24.5%、2.5%和68.9%、28.9%和2.2%,两组之间差异无统计学意义(χ~2=1.27,P=0.53);A等位基因在两组间的分布频率分别是14.7%和16.7%,两组之间差异无统计学意义(χ~2=0.72,P=0.40)。rs1137101位点上GG、GA和AA基因型在糖尿病组和正常对照组之间的分布频率分别为77.6%、21.2%、1.2%和77.8%、21.1%、1.1%,两组之间差异无统计学意义(χ~2=0.02,P=0.99);A等位基因在两组间的分布频率分别是11.8%和11.7%,两组之间差异无统计学意义(χ~2=0.01,P=0.94)。Logistic回归分析结果表明,与GG型研究对象相比,rs1137100位点GA型、AA型研究对象发生糖尿病的风险相似,OR值分别为1.06(95%CI 0.34~3.34)和0.80(95%CI 0.54~1.19);在rs1137101位点上,与GG型研究对象相比,GA型、AA型研究对象发生糖尿病的风险相似,OR值分别为1.12(95%CI 0.22~5.63)和1.01(95%CI 0.66~1.54)。结论瘦素受体基因rs1137100和rs1137101位点的变异与汉族老年人糖尿病没有明显的相关性。

Objective To explore the relationship of diabetes in older Han adults with leptin receptor gene rs1137100 and rs1137101 single nucleotide polymorphism （SNP）. Methods A total of 511 older Han adults were selected for this study and divided into diabetes and control groups, with 241 and 270 people in each group. The genotypes of rsl137100 and rs1137101 and the allele frequency were determined by Taqman method. The correlations between genotypes of leptin receptor gene SNPs with diabetes were analyzed. Blood concentrations of lipids, fasting serum glucose （FBG）, leptin and insulin were also measured. Results The distribution of GG, GA and AA genotypes in rs1137100 in the diabetes group and the healthy group was 73.0% , 24.5% , 2.5% and 68.9% , 28.9% , 2.2% , respectively. The difference was not significant between the two groups（χ^2 = 1.27 ,P =0.53）. There was no significance in the difference of the frequency of ＂A＂ allele in rsl137100 in the two groups, with 14.7% and 16.7% in each group （χ^2 =0.72,P =0.40）. In rsl137101, the distribution of GG,GA and AA genotypes in the diabetes group and the healthy group was 77.6%, 21.2%, 1.2% and 77.8%, 21.1% , 1.1% , respectively. The difference was not significant between the two groups （χ^2 = 0. 02,P = 0.99）. There was no significance in the difference of the frequency of ＂A＂ allele in rsl137101 in the two groups, with 11.8% - 11.7% in each group（χ^2 = 0.01,P = 0.94. The Logistic analysis showed that GA and AA genotypes carriers in rs1137100 had similar risk for diabetes when comparing with the GG genotypes （OR = 1.06, 95% CI 0.34 - 3.34, OR = 0.80,95% CI 0.54 - 1.19）. The GA and AA genotypes carriers in rs1137101 had similar risk for diabetes when comparing with the GG genotypes, and the OR values were 1. 12 （95% CI O. 22 -5.63） and 1.01 （95% CI 0.66 - 1.54） , respectively. Conclusion The rsl137100 and rsl137101 polymorphism are not significantly associated with the prevalence of diabetes in older Han adults.