有序胶原材料联合CBD-BDNF对大鼠脊髓损伤的修复作用

Uniaxial-channel collagen scaffolds loaded with CBD-BDNF improves the recovery of rat spinal cord injury

目的联合有序胶原支架材料和胶原结合结构域-脑源性神经营养因子(CBD-BDNF)修复大鼠脊髓损伤。方法以大鼠脊髓半横断损伤模型观察有序材料联合CBD-BDNF对脊髓损伤的修复效果。大鼠分为3组:Control组即损伤后自动恢复组、Collagen组即损伤部位植入有序材料组和CBD-BDNF/collagen组(CBC组)即损伤部位植入有序胶原材料联合CBD-BDNF组。通过神经丝免疫荧光染色、BBB评分和斜板实验观察修复效果。结果体外实验发现,有序材料能够引导神经元细胞方向性延伸轴突;体内实验发现,CBC组再生神经丝阳性的神经突起数量明显比Control组和Collagen组多(P<0.05),且再生神经丝延伸具有方向性。CBC组大鼠术后9周BBB评分为8(7,13),明显高于Control组[1(1,1)]和Collagen组[2(1,2)](P<0.05)。CBC组大鼠斜板耐受角度也要高于Control组和Collagen组(P<0.05)。结论 CBD-BDNF联合有序胶原支架材料促进脊髓损伤部位神经突起再生和方向性延伸,促进大鼠运动功能恢复,为临床上治疗脊髓横断损伤提供一定实验依据。

Objective To explore the efficacy of uniaxial guidance collagen scaffolds loaded with collagen binding do- main-brain derived neurotrophic factor （CBD-BDNF） in the repair of rat spinal cord injury （SCI）. Methods Rat he- misection SCI models were established and divided into 3 groups： control group, collagen group, and CBD-BDNF/col- lagen （CBC） group. Nerve fiber immunofluorescence staining, BBB score, and inclined plate test were adopted to evaluate the repair efficacy. Results Axons grew through collagen scaffolds in linear fashion in vitro. In animal exper- iments, CBC group displayed more regenerated neurofilament with linear direction than the other two groups （P 〈 0.05 ）. Nine weeks after implantation, the BBB score of CBC group reached 8 （7, 13 ）, significantly higher than the control group E 1 （ 1, 1 ） ] and collagen group E 2 （ 1, 2） ], （ P 〈 0.05 ）. Inclined plane results also showed significant improvement of functional recovery after SCI in CBC group than the other two groups （P 〈 0.05 ）. Conclusion Colla- gen scaffolds loaded with CBD-BDNF significantly improves the SCI recovery evaluated by the BBB scale, inclined plane test and immunohistochemical staining with antineurofilament antibody, which suggests that uniaxial guidance collagen scaffolds loaded with CBD-BDNF may serve as an effective strategy for SCI repair.