两株筛自大规模生产跑道池的节旋藻性能比较研究

Comparative Characterization of Two Arthrospira Strains Isolated from Full-scale Raceway Pond

温度是影响节旋藻大规模培养中生物质产率的重要因素。筛选高产和耐受温度胁迫的优良藻株对提高节旋藻产量具有重要意义。从生产规模跑道池中分离到形态特征差异显著而亲缘关系很近的两株节旋藻Arthrospira sp.DICP-D(D)和Arthrospira sp.DICP-F(F),对其生物质积累性能和对低温、高温胁迫的耐受能力进行了评估。结果显示,尽管藻株D和藻株F在正常条件下拥有相似的生物质组成,在N胁迫和低温胁迫下拥有相同的碳水化合物积累能力,然而藻株D的生物质产率比相同条件下的藻株F高33%~230%。藻株D对高温胁迫的耐受能力是藻株F的2.1倍。藻株D在41℃和15℃下的生物质产率分别维持在正常温度下的73%和61%,显示其对温度胁迫的良好耐受能力。藻株D比藻株F拥有更有效的光保护机制,使其能够更好地适应胁迫环境。藻株D在生物质产率和胁迫耐受能力上的优良性状使其在户外大规模培养中具有良好的应用前景。

Temperature is an important factor affecting biomass productivity in large-scale cultivation of Arthrospira. To improve Arthrospira production, it is crucial to screen strains with high productivity and excellent temperature-tolerance ability. Two strains Arthrospira sp. DICP-D （D） and Arthrospira sp. DICP-F （F） were isolated and identified from full-scale Arthrospira cultivation raceway pond. Although strain D and strain F displayed significant difference in morphology, they shared closest phylogenetic relationship among the existing Arthrospira strains with accessible 16S rRNA gene information. Under normal conditions, strain D and strain F harbor almost the same biomass compositions, and they also accumulated identical carbohydrate content in cell under nitrogen stress or low temperature stress conditions. However, the biomass productivity in strain D was 33% to 230% higher than that in strain F under the same cultivation conditions tested. Strain D showed 1. 1 times better tolerance ability towards high temperature stress than strain F. The biomass productivity in strain D under 41℃ and 15℃ could be maintained at 73% and 61%, respectively, of that under normal temperature, demonstrating that strain D had good tolerance ability towards temperature stress. Strain D held more efficient photoprotection mechanism than strain F did, which endowed it better ability to acclimate to stressful conditions. The excellent biomass productivity and stress tolerance ability in strain D could make it applicable with great potential in outdoor large-scale Arthrospira cultivation.