摘要
Our study aimed to investigate the protective effects of Holothurian intestines(HI) on NSAIDs-induced gastric mucosal damage and the possible mechanism. At first, 60 male Wistar rats were induced of gastric lesions with indomethacin(IDM, 30 mg kg^(-1)). The rats were pretreated for 15 consecutive days with saline, sucralfate, or HI(0.4g kg^(-1) d-1, 0.8 g kg^(-1) d^(-1) and 1.6 g kg^(-1) d^(-1)) prior to IDM treatment, followed by evaluations of macroscopic damage and microscopic features; and investigation of the levels of inflammatory cytokines, oxidative stress parameters, gastric mucosal prostaglandin E2(PGE2) and total hexosamine in tissues. The expression of COX-1 and COX-2 m RNA in the gastric tissue were determined by quantitative polymerase chain reaction(q PCR). Pathological gastric ulcer indexes, levels of pro-inflammatory cytokines(IL-1β, IL-17, TNF-α) and lipid peroxidation were significantly decreased in HI-treated groups, whereas the levels of protective factors(TGF-β, GSH, SOD activity and PGE2) were significantly elevated especially in the group with HI 1.61 g kg^(-1) d^(-1)(P < 0.05). Furthermore, the expression of COX-2 mRNA decreased significantly in HI groups(P < 0.05). The study investigates that holothurian intestines may act as a kind of marine medicine which have protective effect on IDM-induced gastric ulcer, which could be a dietary preventive agent for the prevention of gastric damage.
Our study aimed to investigate the protective effects of Holothurian intestines（HI） on NSAIDs-induced gastric mucosal damage and the possible mechanism. At first, 60 male Wistar rats were induced of gastric lesions with indomethacin（IDM, 30 mg kg^-1）. The rats were pretreated for 15 consecutive days with saline, sucralfate, or HI（0.4g kg^-1 d^-1, 0.8 g kg^-1 d^-1 and 1.6 g kg^-1 d^-1） prior to IDM treatment, followed by evaluations of macroscopic damage and microscopic features; and investigation of the levels of inflammatory cytokines, oxidative stress parameters, gastric mucosal prostaglandin E2（PGE2） and total hexosamine in tissues. The expression of COX-1 and COX-2 m RNA in the gastric tissue were determined by quantitative polymerase chain reaction（q PCR）. Pathological gastric ulcer indexes, levels of pro-inflammatory cytokines（IL-1β, IL-17, TNF-α） and lipid peroxidation were significantly decreased in HI-treated groups, whereas the levels of protective factors（TGF-β, GSH, SOD activity and PGE2） were significantly elevated especially in the group with HI 1.61 g kg^-1 d^-1（P 〈 0.05）. Furthermore, the expression of COX-2 mRNA decreased significantly in HI groups（P 〈 0.05）. The study investigates that holothurian intestines may act as a kind of marine medicine which have protective effect on IDM-induced gastric ulcer, which could be a dietary preventive agent for the prevention of gastric damage.
基金
supported by the Pharmacology Laboratory of Qingdao University
funding from the Dalian Bangchui Island Seafood Co., Ltd.
Shandong Medical and Health Science and Technology Development Plan (No. 2013WS0270)
Natural and Science Funding of Shandong Province (ZR2014HM094)
College and University Scientific Research Development Program of Shandong Province (J15LL5T)
Qingdao People's Livelihood Science and Technology Plan (14-2-3-8-nsh)
