CD44和CD133在口腔潜在恶性病变和口腔鳞状细胞癌中的表达及其临床意义

Clinical significance of CD44 and CD133 expression in oral potentially malignant disorder and oral squamous cell carcinoma

目的探究肿瘤干细胞标记物CD44和CD133在口腔正常黏膜、口腔潜在恶性病变(OPMD)及口腔鳞状细胞癌(OSCC)中的表达情况及相互关系,评估其作为OPMD恶性转化早期诊断指标的临床价值。方法回顾性分析60例OPMD患者、60例OSCC患者及10例口腔黏膜正常者的临床资料,采用免疫组织化学双重染色技术检测各组病理组织中CD44与CD133的表达,分析CD44和CD133表达之间的关系及其与各临床因素之间的关系。结果口腔正常黏膜、OPMD、OSCC三组中,CD44的阳性表达率分别为100.00%、96.67%、71.67%(P<0.05),CD133的阳性表达率分别为0.00%、35.00%、63.33%(P<0.05)。CD44与CD133二者间的表达具有相关性(P<0.05),且其表达又都与OSCC的临床分期和淋巴结转移相关(P<0.05)。结论 CD44和CD133作为评估OPMD恶性转化潜能的指标具有重要临床价值。

Objective This study aims to investigate the expression and relationship of CD44 and CD133 in normal oral mucosa, oral potentially malignant disorder （OPMD）, and oral squamous cell carcinoma （OSCC）. This work also analyzes the relationship between such expression and clinical factors. This study intends to evaluate the clinical value of using CD44 and CD133 as indices to evaluate the carcinogenic potential of OPMD. Methods Clinical data from 60 patients with OPMD, 60 patients with OSCC, and 10 cases of normal oral mucosa were analyzed. Double immunohistochemical analysis was applied to investigate the expression of CD44 and CD133 in paraffin sections of normal oral mucosa, OPMD, and OSCC tissues. Subsequently, the relationships between such expression and clinical factors were analyzed. Results The positive rates of CD44 expression in the normal oral mucosa, OPMD, and OSCC tissues were 100.00%, 96.67%, and 71.67% （P〈0.05）, res- pectively. Meanwhile, the positive rates of CD133 expression in the normal oral mucosa, OPMD, and OSCC tissues were 0.00%, 35.00%, and 63.33% （P〈0.05）, respectively. The expression of CD44 and CD133 was found to be correlated （P〈0.05）. Such expression was related to the clinical stages and lymphatic metastasis ofOSCC （P〈0.05）. Conclusion CD44 and CD133 can be used individually as clinical indices to evaluate the carcinogenic potential of OPMD.