干扰素刺激基因15抑制严重发热伴血小板减少综合征病毒复制的初步研究

Study on the role of interferon-stimulated gene 15(ISG15) to inhibit the replication of severe fever with thrombocytopenia syndrome virus

目的了解干扰素刺激基因15(ISG15)对严重发热伴血小板减少综合征病毒(SFTSV)复制的影响。方法1)ISG15基因克隆:通过逆转录聚合酶链式反应(RT-PCR)的方法,从Hela细胞中扩增获得ISG15基因,然后连接质粒形成ISG15表达载体。2)研究ISG15表达对SFTSV复制的影响:将ISG15质粒转染Hela细胞并过表达后,接种SFTSV,随后用荧光定量RT-PCR的方法观察ISG15对SFTSV复制的影响。结果成功构建ISG15表达质粒,并在Hela细胞内过表达;SFTSV成功在Hela细胞内增殖,同时过表达ISG15可以抑制SFTSV的复制。结论初步证明在Hela细胞中,过表达ISG15可以抑制SFTSV的复制,但具体机制还有待进一步探讨。

Objective To analyze the impact of interferon-stimulated gene 15 （ISG15） on the replication of SFTSV.Methods To construct a plasmid with over-expression of ISG15,ISG15 gene was amplified from Hela cell using reverse transcription （RT）-PCR and then cloned in the plasmid.The effect of ISG15 expression on SFTSV replication was evaluated by transfecting over-expressed ISG15 plasmid on Hela cells.The effect of ISG15 over-expression on SFTSV replication was evaluated after infection with SFTSV using quantitative RT-PCR.Results ISG15 was expressed successfully in Hela cells.Hela cell supported the replication of SFTSV.The over-expression of ISG15 might inhibit the replication of SFTSV.Conclusion The over-expressed ISG15 can inhibit the replication of SFTSV.However,its mechanism needs to be further explored.