sEng、sFIt-1及AT1-AA在子痫前期患者中的表达及诊断价值

The expression and clinical significance of sEng,sFIt-1,AT1-AA in patients with preeclampsia

目的探讨可溶性内皮因子(sEng)、可溶性Fms样酪氨酸激酶-1(sFlt-1)、血管紧张素Ⅱ型受体自身抗体(AT1-AA)在子痫前期患者中的表达及其在子痫前期诊断中的应用价值。方法选择2015年1-12月85例子痫前期患者(其中轻度子痫前期45例,重度子痫前期40例)及80例妊娠健康孕妇(对照组),应用ELISA测定两组孕妇外周血及新生儿脐血中sEng、sFIt-1、AT1-AA水平。应用Pearson相关分析各指标的相关性,应用受试者工作特征曲线(ROC)分析sEng、sFIt-1、AT1-AA在子痫前期中的诊断价值。结果重度子痫前期组及轻度子痫前期组孕妇外周血及新生儿脐血中sEng、sFIt-1、AT1-AA水平均高于对照组(P<0.05),而重度子痫前期组外周血及新生儿脐血中sEng、sFIt-1、AT1-AA水平均高于轻度子痫前期组(P<0.05)。经Pearson相关分析可知,sEng与sFIt-1、AT1-AA呈正相关(P<0.05),sFIt-1与AT1-AA呈正相关(P<0.05)。经ROC曲线分析可知,sEng、sFIt-1、AT1-AA在子痫前期中诊断灵敏度及特异度分别为76.3%和85.5%、79.2%和85.6%、70.8%和82.2%。结论子痫前期孕妇外周血、脐血中sEng、sFIt-1、AT1-AA与子痫前期的发生及进展有密切的关系,可用于子痫前期诊断,同时可作为子痫前期发生及进展的预测指标。

Objective To investigate the expression and clinical significance of soluble endothelial growth factor （sEng）,soluble Fms-like tyrosine kinase-1 （sFlt-1）,angiotensin Ⅱ receptor autoantibodies （AT1-AA） in patients with preeclampsia.Methods A total of 85 patients with preeclampsia （45 cases with mild preeclampsia,40 cases with severe preeclampsia） and 80 cases of healthy pregnant women （control group） were selected from January 2015 to December 2015.The levels of sEng,sFIt-1 and AT1-AA were detected by ELISA.The correlation of each index was analyzed with Pearson correlation.The diagnostic values of sEng,sFIt-1 and AT1-AA in preeclampsia were analyzed by receiver operating characteristic （ROC）.Results The levels of sEng,sFIt-1 and AT1-AA of maternal blood and cord blood of newborns of severe preeclampsia group and mild preeclampsia were significantly higher than of control group （P〈0.05）,and the levels of sEng,sFIt-1 and AT1-AA of severe preeclampsia group were significantly higher than that of mild preeclampsia group （P〈0.05）.The level of sEng was positively correlated with sFIt-1 and AT1-AA （P〈0.05）,sFIt-1 was positively correlated with AT1-AA （P〈0.05）.The sensitivity and specificity of sEng,sFIt-1,AT1-AA were 76.3% and 85.5%,79.2% and 85.6%,70.8% and 82.2% by ROC analysis.Conclusion Maternal blood,cord blood sEng,sFIt-1 and AT1-AA in patients with preeclampsia are closely related with preeclampsia and progress,these indicators can be used to diagnose of preeclampsia,as well as predictors of preeclampsia and progress.