摘要
The fruits of Paulownia catalpifolia Gong Tong are used as a Chinese folk herbal medicine for the treatment of enteritis, tonsillitis, bronchitis, and dysentery, etc. Our previous study has identified new C-geranylated flavanones with obvious anti-proliferative effects in lung cancer A549 cells. In the present study, a new C-geranylated flavone, paucatalinone C(1) and five known C-geranylated flavanones(2-6) were isolated. In addition, a total of 34 C-geranylated flavonoids were detected by HPLC-DAD-ESI-MS/MS coupling techniques from the CH_2Cl_2 extract of P. catalpifolia. Futhermore, anti-aging effects of isolated compounds were evaluated in vitro with premature senescent 2BS cells induced by H_2O_2. Phytochemical results indicated that P. catalpifolia was a natural resource of abundant C-geranylated flavonoids. Diplacone(3) and paucatalinone A(5) were the potent anti-aging agents in the premature senescent 2BS cells induced by H_2O_2 and the C-geranyl substituent may be an important factor because of its lipophilic character.
The fruits of Paulownia catalpifolia Gong Tong are used as a Chinese folk herbal medicine for the treatment of enteritis, tonsillitis, bronchitis, and dysentery, etc. Our previous study has identified new C-geranylated flavanones with obvious anti-proliferative effects in lung cancer A549 cells. In the present study, a new C-geranylated flavone, paucatalinone C（1） and five known C-geranylated flavanones（2-6） were isolated. In addition, a total of 34 C-geranylated flavonoids were detected by HPLC-DAD-ESI-MS/MS coupling techniques from the CH_2Cl_2 extract of P. catalpifolia. Futhermore, anti-aging effects of isolated compounds were evaluated in vitro with premature senescent 2BS cells induced by H_2O_2. Phytochemical results indicated that P. catalpifolia was a natural resource of abundant C-geranylated flavonoids. Diplacone（3） and paucatalinone A（5） were the potent anti-aging agents in the premature senescent 2BS cells induced by H_2O_2 and the C-geranyl substituent may be an important factor because of its lipophilic character.
基金
financially supported by the Natural Science Foundation of Shandong Academy of Medical Sciences(No.2015-08)
the Innovation Project of Shandong Academy of Medical Sciences and the Natural Science Open Foundation of State Key Laboratory of Bioactive Substance and Function of Natural Medicines(No.GTZK201503)
