DNJ对正常小鼠脂代谢的影响及作用机理初探

Effect of 1-Deoxynojirimycin on Lipid Metabolism in Normal Mice and Preliminary Exploration of the Underlying Mechanism

本文为探索DNJ对脂代谢的影响及作用机理,给正常小鼠灌胃DNJ 40 d后,用试剂盒测定各实验动物脂代谢相关生化指标、酶活及抗氧化指标。结果显示与阴性对照组比较,8.0 mg/(kg bw?d)剂量DNJ使雌、雄小鼠的体重增加率分别降低59.40%和9.15%;腹腔脂肪系数降低38.30%和15.18%;肝脏脂肪含量降低18.42%和36.66%;血清TC降低27.40%和28.57%,TG降低0.89%和15.03%,HDL增加42.19%和6.80%;肝脏SOD活性上升29.24%和25.17%,GSH增加16.29%和15.00%,8-isoprostane下降18.53%和15.19%;雌鼠FAS、ACO活性下降14.02%和9.67%,雄鼠ACO活性上升21.63%。说明适量DNJ主要通过抑制雌鼠脂肪酸合成、加速雄鼠脂肪酸氧化来减少脂肪蓄积,且对雌鼠具有更好的预防肥胖作用;同时可增强小鼠机体抗氧化能力,预防脂代谢异常。

To explore the effect of 1-deoxynojirimycin（DNJ） on the lipid metabolism, and the corresponding mechanism, normal mice were fed DNJ by gavage for 40 days, and then biochemical indicators of lipid metabolism, the activity of enzymes, and the antioxidant indices of the animals were determined using enzyme-linked immunosorbent assay（ELISA） kits. Compared with the negative control group, 8.0 mg/kg.bw/d dosage of DNJ decreased the rates of weight gain by 59.40%（female） and 9.15%（male）, decreased intraperitoneal fat coefficients by 38.30%（female） and 15.18%（male）, and decreased the contents of liver fat by 18.42%（female） and 36.66%（male）The addition of DNJ also decreased the contents of serum total cholesterol（TC） by 27.40%（female） and 28.57%（male）, and decreased the contents of triglycerides（TG） in serum by 0.89%（female） and 15.03%（male）. Besides, this dose increased the contents of high-density lipoproteins（HDL） in serum by 42.19%（female） and 6.80%（male）, increased the activities of superoxide dismutase（SOD） in liver by 29.24%（female） and 25.17%（male） increased the contents of glutathione（GSH） in liver by 16.29%（female） and 15.00%（male）, and decreased the contents of 8-isoprostane in liver by 18.53%（female） and 15.19%（male）. It was also found that 8.0 mg/（kg bw？d） dosage of DNJ increased the activities of hepatic fatty acid synthase（FAS） and acyl-Co A oxidase（ACO） in female mice by 14.02% and 9.67%, respectively, and increased the activity of ACO in the liver of male mice by 21.63%. The results indicated that an appropriate dosage of DNJ could reduce fat accumulation by inhibiting fatty acid synthesis in female mice and accelerating fatty acid oxidation in male mice; a better effect on obesity prevention was shown in female mice. DNJ could also enhance the body’s antioxidant ability to prevent abnormal lipid metabolism.