桑叶-苦瓜混合粉对糖尿病小鼠的降糖作用

Hypoglycemic Effect of Mulberry Leaf-balsam Pear Mixed Powder on Diabetic Mice

本文研究了桑叶-苦瓜混合粉对糖尿病小鼠的降糖作用,为糖尿病保健食品的开发提供参考。采用腹腔注射四氧嘧啶复制糖尿病小鼠模型,造模成功后分为6组,分别是模型组、阳性对照组(二甲双胍组)和桑叶-苦瓜混合粉4个剂量组,并设空白对照组,对小鼠喂饲基础饲料和桑叶-苦瓜混合粉,测定小鼠的体重、糖耐量、肝糖原以及血糖含量变化。结果表明,给糖尿病小鼠灌喂浓度为0.20 g/m L,比例为3:1桑叶-苦瓜混合粉结合喂饲基础饲料30 d,与模型对照相比,雌、雄小鼠体重下降受到抑制,雌、雄小鼠体重下降的抑制率分别达到其69.44%和70.31%;血糖分别下降45.8%和42.99%;血糖时间曲线下面积(AUC)分别下降29.77%和29.54%;肝糖原分别增加了1.42和1.50倍。所以在控制饮食的同时摄入一定剂量的桑叶-苦瓜混合粉,可以对糖尿病小鼠起到良好的辅助降糖作用。

The possible hypoglycemic effects of mulberry leaf-balsam pear mixed powder fed to diabetic mice were investigated to provide a reference for the development of healthy foods for patients with diabetes. After a mouse model of diabetes was successfully created by intraperitoneal injection of alloxan, the mice were divided into six groups： one model group, four groups receiving different doses of mulberry leaf-balsam pear mixed powder, and a positive control group（metformin）; an untreated control group was also included. The mice were fed basal feed and mulberry leaf-balsam pear mixed powder, and the body weight, glucose tolerance, liver glycogen content, and changes of blood glucose were measured. The results show that compared with model group, female and male diabetic mice that were fed 0.20 g/m L 3：1 mulberry leaf-balsam pear mixed powder and basal feed for 30 d experienced an inhibition of weight loss, and the inhibition rates reached 69.44% and 70.00%, respectively. For female and male mice, the blood glucose levels were decreased by 45.8% and 42.99%, respectively, the areas under the blood glucose time curve（AUC） were decreased by 29.77% and 29.54%, respectively, and liver glycogen levels were increased by 1.42 and 1.50 times, respectively. With dietary control, an intake of a certain dose of mulberry leaf-balsam pear mixed powder can exert a good auxiliary hypoglycemic effect on diabetic mice.