Hox转录反义RNA、zeste基因增强子同源物2与血管内皮生长因子在鼻咽癌组织中的表达及其预后相关性研究

Expression of Hox transcript antisense RNA,enhancer of zeste homolog 2 and vascular endothelial growth factor in nasopharyngeal carcinoma tissues and their correlation with prognosis

目的初步探讨Hox转录反义RNA(Hox transcript antisense RNA,HOTAIR)在鼻咽癌中与zeste基因增强子同源物2(enhancer of zeste homolog 2,EZH2)及血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达情况及其与鼻咽癌患者预后的相关性。方法检测HOTAIR、EZH2、VEGF在鼻咽癌患者中的表达情况并分析HOTAIR的表达与鼻咽癌患者临床特征的相关性。结果鼻咽癌组织中HOTAIR、EZH2及VEGF在mRNA水平及蛋白水平(U=955,P<0.05)均明显高于慢性炎症组织,差异具有统计学意义;相关分析显示鼻咽癌组织中HOTAIR与VEGF表达量呈正相关(r=0.599,P<0.001),VEGF与EZH2呈正相关(r=0.369,P=0.012),而HOTAIR与EZH2无明显相关性(r=0.139,P=0.357);HOTAIR高表达的鼻咽癌患者,其N分级、M分级及临床分期较高,近期疗效较差。结论鼻咽癌患者中HOTAIR及EZH2、VEGF蛋白的表达均高于慢性炎症组,HOTAIR可能通过VEGF、EZH2发挥其促进肿瘤转移的作用,并与鼻咽癌预后相关。

OBJECTIVE To explore the expression of long non-coding RNA （LncRNA） HOTAIR, enhancer of Zeste homolog 2 （EZH2） and vascular endothelial growth factor （VEGF） in nasopharyngeal carcinoma （NPC） and their relationship between HOTAIR and prognosis. METHODS We examined the expression of HOTAIR, EZH2 and VEGF in NPC tissues, and analyzed the clinical significance of HOTAIR expression in patients with NPC. RESULTS We found that the expression of HOTAIR, EZH2 and VEGF in the NPC tissues were significantly higher than those in the chronic nasopharyngitis tissues in the gene and protein levels （U=955, P〈0.05）. The HOTAIR was positively correlated with VEGF （r=0.599, P〈0.001）, VEGF was positively correlated with EZH2 （r=0.369, P=0.012）, and HOTAIR had no significant correlation with EZH2 （1＂=0.139, P=0.357）. Moreover, HOTAIR expression levels increased with clinical stage progression. CONCLUSION The expression of HOTAIR, EZH2 and VEGF were significantly increased in the NPC tissues, and the expression of HOTAIR was related with the TNM stage, the clinical stage and the short term therapeutic effect of the NPC patients, which indicated that the HOTAIR might be used as a biological indicator to determine the prognosis of the NPC.