摘要
目的:对丹参精准煮散饮片进行质量评价,为临床用药安全有效提供保障。方法:应用第二内转录间隔区(ITS2)序列作为DNA条形码对丹参进行鉴定,采用化学指纹图谱表征药材化学组成,评价丹参原饮片及精准煮散饮片的相似度,测定指标成分丹酚酸B和迷迭香酸的含量,标定指纹图谱共有峰。采用标准汤剂煎煮法比较原饮片及精准煮散饮片的煎煮效率,对煮散体系进行综合评价。结果:ITS2序列对丹参药材可实现准确鉴定;原饮片及煮散饮片煎出成分基本无变化,指纹图谱相似度均很高,达到0.99以上,但是煮散饮片煎煮效率明显增高。按标准汤剂煎煮法,煮散饮片出膏率增加>30%,迷迭香酸及丹酚酸B的煎出率均约1.7倍,其余化学成分的煎出率为原饮片的1.2~2.2倍;且精准煮散饮片差异明显小于原饮片批间差异。结论:丹参精准煮散饮片能提高原饮片的煎煮效率及均一性,具有较好的推广与应用价值。
Objective: To investigate the rationality and superiority of precise powder decoction pieces system, Salviae Mihiorrhizae Radix et Rhizoma was employed in this study. Method: The second internal transcribed spacer (ITS2) sequence was used as a DNA barcode to identify Salviae Miltiorrhizae Radix et Rhizoma. The chemical composition of the medicinal materials was characterized by HPLC fingerprints, similarity between precise powder decoction pieces and traditional commercial slices were both evaluated. The eoncents of salvianolic acid B and rosmarinic acid were determined by HPLC, and the characteristic common peaks were identified. In addition, the extraction efficiency was also compared by standard decoction method. Result: Salviae Miltiorrhizae Radix et Rhizoma could be accurately identified by ITS2 sequence. HPLC fingerprints were very similarity between precise powder decoction pieces and traditional commercial slices. However, the extraction efficiency of precise powder decoction pieces was significantly improved with 〉 30% increased dry extract yield and approximately 20%-120% increased dissolution of chemical components. Moreover, the relative standard deviation was also decreased between batches. Conclusion: Precise powder decoction pieces can improve the extraction efficiency and uniformity of traditional commercial slices.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第11期18-22,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省中医院专项(2015KT1817)
中国中医科学院中医药健康服务专项(ZZ0908067)
广东省中医药强省建设专项医院中医药制剂开发项目(2015KT1535)
关键词
精准煮散饮片
丹参
指纹图谱
均一性
DNA条形码
丹酚酸B
迷迭香酸
precise powder decoction pieces
Salviae Miltiorrhizae Radix et Rhizoma
fingerprints
uniformity
DNA barcode
salvianolic acid B
rosmarinic acid