盐胁迫下木炭对小麦幼苗根系蛋白表达的影响

Effect of Charcoal on Protein Expression of Wheat Seedling Root under Salt Stress

为明确木炭对小麦盐胁迫的缓解效应,以冬小麦品种临汾6339为材料,对小麦幼苗分别进行盐和木炭单因素处理及二者复合处理,应用蛋白质双向电泳与质谱联用技术对小麦根系差异表达蛋白进行了分析。结果表明,相对盐处理,在盐和木炭复合处理的小麦根系中共检测到丰度变化在2倍以上的差异表达蛋白点12个。经过MALDI-TOF-TOF分析及数据库检索,被鉴定的12个蛋白点按其功能大致可归为5类。其中,第Ⅰ类为与能量代谢相关的蛋白质,包括ATP合酶和ATP酶;第Ⅱ类为与糖代谢相关的蛋白质,包括磷酸甘油醛异构酶、磷酸丙糖异构酶、6-磷酸葡糖酸脱氢酶、苹果酸脱氢酶以及乌头酸水合酶;第Ⅲ类为与氨基酸代谢相关的蛋白质,如半胱氨酸合成酶;第Ⅳ类为与遗传物质有关的蛋白质,如染色体的结构蛋白;第Ⅴ类为未知功能蛋白质。

Winter wheat Linfen 6339 was used to determine the effect of charcoal on wheat seedling root under salt stress. The wheat seedlings were treated with single factor （salt or charcoal） and two factors （salt and charcoal）. The differential expression proteins in wheat roots were analyzed by two- dimensional eleetrophoresis and mass spectrometry. The results showed that compared with single factor treatment,with the increase of NaC1 and charcoal concentration,a total of 12 differentially expressed protein spots were detected in the root system of wheat with abundance variation more than 2 times. The identified 12 protein spots could be classified into 5 categories according to their functions through MALDI-TOF-TOF analysis. Among them, ATP synthase and ATP enzymes belonged to Class I ,which are relative to energy metabolism. Class Ⅱ contained sugar metabolism related proteins,including phosphoglycerate kinase, triosephosphateisomerase, 6-phosphogluconate dehydrogenase, malic enzyme and aconitatehydratase. Class contained cysteine synthase belonging to amino acid metabolism related proteins. Class Ⅳ contained structural proteins of chromosome, belonging to hereditary substance related proteins. Class Ⅴ contained unknown function proteins.