组织工程支架材料性质对干细胞分化的影响

Effects of scaffold material properties on stem cell differentiation in tissue engineering

背景:许多研究证实了生物材料的物理性质尤其是其硬度能够调控干细胞向目的细胞定向分化。目的:综述细胞外基质对干细胞行为的影响及生物材料物理性质调控干细胞分化的最新研究进展。方法:由第一作者检索中国期刊全文数据库和Web of Science数据库,检索词分别为"细胞外基质、生物支架材料、化学性质、物理性质、基质硬度、干细胞分化"和"extracellular matrix,biomaterials scaffolds,chemical properties,physical properties,substrate rigidity,stem cell differentiation"。语言分别设定为中文和英文,最终选择31篇文献进行综述。结果与结论:生物材料支架的物理性质能够影响细胞的增殖、迁移和分化等行为,尤其是硬度调控干细胞分化对组织工程及再生医学都具有极大的启示。寻找更多与细胞外基质硬度共同作用的生物化学因素和物理因素,精确控制细胞行为,制备出与体内生理环境更加相似的支架材料值得深入研究和探讨。

BACKGROUND： Increasing evidences support that the physical properties, especially stiffness, can regulate the directional differentiation of stem cells. OBJECTIVE： To review the effects of extracellular matrix on stem cell behaviors, and the research progress concerning the influence of physical properties of biomaterials on stem cell differentiation. METHODS： The first author retrieved CNKI and Web of Science databases using the keywords of ＂extracellular matrix, bioscaffolds materials, chemical property, physical property, substrate rigidity, stem cell differentiation＂ in Chinese and English, respectively. Finally, a total of 31 literatures were included for analysis. RESULTS AND CONCLUSION： The physicochemical properties expose effects on the cell proliferation, migration and differentiation, and especially the regulatory effect of stiffness on the cell differentiation has a revelation for tissue engineering and regenerative medicine. Searching for more biochemical and physical factors interacting with the stiffness of extracellular matrix will enable us to control cell behaviors accurately and to prepare an ideal scaffold closely similar to in vivo environment.