122味维吾尔药体外抗真菌作用

Antifungal activity of 122 kinds of Uighur medicines in vitro

目的测定122味维吾尔药的体外抗真菌活性,以期找到活性较好的抗真菌药物。方法用75%乙醇超声提取维吾尔药;美国临床实验室标准化协会(CLSI)推荐的M38-A2和M27-A3微量液基稀释法进行抗深部真菌活性筛选;棋盘微量液基法考察维吾尔药与氟康唑协同抗耐药白假丝酵母菌的作用;磷钼钨酸-干酪素法测定鞣质含量,并比较不同溶剂提取部位抑菌活性与鞣质含量的关系。结果和结论在122味维吾尔药活性测定中,在2 000μg/m L测定浓度下,对新生隐球菌有抑制作用的有黄诃子皮(Terminalia chebula Retz.)、毛诃子(Terminalia bellerica Roxb.)等21味,对白假丝酵母菌有抑制作用的有黄诃子皮、毛诃子等15味,对烟曲霉仅姜黄(Curcuma longa L.)有效。姜黄抗菌谱最广,且对深部真菌及浅部真菌均有较好的抑制作用。9味维吾尔药与氟康唑合用对耐药白假丝酵母菌有较好协同抗真菌作用;黄诃子皮、毛诃子、岩白菜(Bergenia purpurascens Engl.)、阿拉伯胶树汁(Acacia senegal Willd)、鞣树果(Rhus coriaria L.)这5味活性较好药材的抗真菌活性部位集中在乙酸乙酯部位,且不同溶剂提取部位的最低抑菌浓度与鞣质含量呈正相关。

Objective To identify the Uighur medicine with good antifungal activity via determining the in vitro antifungal activities of 122 kinds of Uighur medicines. Methods The 75% ethanol extracts of 122 kinds of Uighur medicines were prepared by ultrasonic extraction, and anti-deep fungal medicines were screened according to the M38-A2 and M27-A3 broth microdilution method approved by the Clinical Laboratory Standards Institute （CLSI）. The broth microdilution checkerboard method was used to determine the synergistic effect of Uighur medicine and fluconazole against drug-resistant Candida albicans. Tannin content was determined by phosphomolybdotungstic acid-casein method, and the relationship between antifungal activity of different extractions and contents of tannin was studied. Results and conclusion At a determination concentration of 2 000 μg/mL, 21 kinds of Uighur medicines such as Terminalia chebula Retz. and Terminalia bellerica Roxb. had inhibitory effect on Cryptococcus neoformans, 15 kinds of Uighur medicines such as Terminalia chebula Retz. and Terminalia bellerica Roxb. had inhibitory effect on Candida albicans, and Curcuma longa L. had inhibitory effect on Aspergillus fumigatus. Curcuma longa L. had the widest antifungal spectrum and showed good inhibitory effect on deep and superficial fungus. Nine kinds of Uighur medicines showed synergistic effects with fluconazole against Candida albicans. Antifungal activity parts of Terminalia chebula Retz., Terminalia bellerica Roxb., Bergenia purpurascens Engl., Acacia senegal Willd and Rhus coriaria L. were concentrated in ethyl acetate, and the minimum inhibitory concentration of different solvent extraction was directly proportional to the content of tannin.