IL-17在药物难治性颞叶外侧型癫痫中的表达

Expression of interleukin 17 in patients with intractable temporal lobe epilepsy

研究白介素17(interleukin 17,IL-17)在药物难治性颞叶外侧型癫痫(lateral temporal lobe epilepsy,LTLE)病灶中的表达和分布,探讨IL-17在TLE发生发展中可能的作用。使用real-time PCR、Western blotting、免疫组化及荧光双标检测12例LTLE致痫灶及8例正常对照脑组织中IL-17的表达。结果提示:IL-17在LTLE中的mRNA和蛋白表达水平较对照组明显增高(P<0.05),且IL-17高表达于LTLE的神经元、星形胶质细胞以及血管内皮细胞。上述研究结果提示,IL-17可能参与了TLE的发生发展。

To explore the effect of IL-I7 on epileptogenesis and development of lateral temporal lobe epilepsy（LTLE）, we investigated the level and expression pattern of IL-17 in patients with intractable LTLE. Real-time PCR and Western blotting revealed upregulated levels of mRNA and protein of IL-17 in the LTLE group compared to the control groups（P〈 0.05）. Immunohistochemistry and double-labeled immunofluorescence showed that IL-17 was highly expressed in neurons, astroeytes and vascular endothelial cells. These findings suggest that IL-17 may contribute to the epileptogenesis and development of TLE.