不同倍性滇杨叶片解剖结构差异分析

Analysis of leaf anatomical structure differences in Populus yunnanensis with different ploidy

以前期诱导获得的5株多倍体滇杨一年生无性系植株为材料,二倍体滇杨为对照,对其叶片进行石蜡包埋、切片,观察其解剖结构差异,测定叶片厚度、栅栏组织厚度、海绵组织厚度,分析多倍化后滇杨叶片的变异特性。结果表明:不同倍性滇杨在叶片解剖结构各性状间均存在不同表现。不同个体间叶片厚度、CTR、SR、上表皮相对厚度、下表皮相对厚度间均存在显著差异,滇杨经多倍化处理后能引起栅栏组织、海绵组织的变化,表现为基因的剂量效应差异。同时,不同倍性滇杨叶片CTR、SR的变化并不与滇杨的倍性成正相关趋势,这为滇杨遗传改良提供了选择的物质基础。研究结果可为滇杨的抗性育种相关性状的早期选择提供理论依据。

Leaves from annual clone individual plant of five polyploid Populus yunnanensis and a diploid plant, as control, were used to embed with paraffin and slice, in order to observe the leaves anatomical structure. The leaf thickness, palisade tissue thickness and spongy tissue thickness were measured to analyze the difference of P.yunnanensis with different ploidy. The results showed that different ploidy Pyunnanensis had the different representation on traits of the leaf anatomical structure. There were significant differences in leaf thickness, CTR, SR, relative thickness of epicuticle, and relative thickness of lower epidermis of Pyunnanensis in different individuals, which indicated that polyploidy could make the palisade tissue and spongy tissue changed, because of gene dose effect. At the same time. The changes on CTR and SR in leaves of Pyunnanensis were not positive correlation with the their ploidy, which offered the selective material basis of Pyunnanensis breeding. Theoretical basis on the early selection of P.yunnanensis resistance breeding could be provided from these results.