麝香酮对外源性骨髓间充质干细胞在颅骨缺损大鼠体内迁移的影响

Effect of musk ketone on in vivo migration of exogenous bone marrow mesenchymal stem cells in skull defect rats

背景:骨髓间充质干细胞是多向分化干细胞,在骨折愈合和中有着重要作用。如何促进其在体内迁移,进而促进骨缺损的修复,有着重要研究意义。目的:研究不同浓度麝香酮对外源性骨髓间充质干细胞在体内迁移的影响,探讨其促进骨折愈合的机制及其引经理论初探。方法:建立大鼠颅骨缺损的大鼠模型;贴壁筛选法提取干细胞,取第3代细胞,利用DAPI进行标记,然后尾静脉回植入颅骨缺损的大鼠体内。分别以0,42,84,168μL/kg麝香酮灌胃1次。结果与结论:与高剂量麝香酮组和空白组相比,低、中剂量麝香酮组骨缺损部分的骨髓间充质干细胞数量增加,干细胞因子和Fractalkine的表达水平明显增加。提示中低剂量麝香酮具有促进外源性干细胞在体内的迁移作用。

BACKGROUND：Bone marrow mesenchymal stem cells （BMSCs） are a kind of stem cells with multi-directional differentiation ability and play an important role in the healing of fractures. Therefore, it is of great significance to explore how to promote the BMSCs migration in vivo, thereby promoting bone defect repair.OBJECTIVE：To study the effects of different concentrations of musk ketone on in vivo migration of exogenous BMSCs, and to preliminarily explore the mechanism of fracture healing and cited theory.METHODS：A rat model of skull defects was made. Passage 3 BMSCs were harvested by using adherence method,labeled with DAPI, and then injected via the tail vein into the model rats. After that, the rats were intragastrically administrated with 0 （blank control）, 42 （low dose）, 84 （middle dose）, 168 μL/kg （high dose） musk ketone, respectively.RESULTS AND CONCLUSION：The number of exogenous BMSCs in the defect region, and the expression of stem cell factor and Fractalkine showed a significant increase in the low- and middle-dose groups compared with the high-dose and blank control groups. These findings indicate that the low- and middle-dose musk ketone can promote the in vivo migration of exogenous stem cells.