通用转录因子3对卵巢癌细胞增殖和凋亡的影响

Effect of General Transcription Factor 3 on Proliferation and Apoptosis of Ovarian Cancer Cells

目的:检测通用转录因子3(BTF3)在卵巢癌生长增殖中的作用,探讨其作为治疗靶点的可行性。方法:构建shBTF3慢病毒载体后转染人卵巢癌SKOV3细胞,采用细胞成像仪检测细胞生长状况,流式细胞术检测细胞周期及凋亡率,MTT法检测慢病毒转染后SKOV3细胞增殖状态的改变。结果:shBTF3慢病毒载体成功转染SKOV3细胞后,与对照组相比,实验组细胞生长状况下降,增殖减慢。SKOV3细胞周期G_1期被抑制,细胞凋亡率升高(P<0.05)。结论:BTF3对卵巢癌细胞SKOV3的生长增殖具有促进作用,考虑BTF3基因在卵巢癌中的表达可能与其发生发展及分级分期密切相关。

Objective： To construct shBTF3 lentivirus transfected SKOV3 cells and to detect its role in the growth and proliferation of ovarian cancer. Methods： We successfully constructed shBTF3 lentiviral vector to transfect SKOV3 cells. The growth status of the cells was detected by cell imaging, the ceil cycle and apoptosis rate were detected by flow cytometry, and the proliferation of SKOV3 cells was detected by MTT assay. Results： Compared with the control group, shBTF3 lentiviral vector successfully transfected SKOV3 cells, the growth of cells in the experimental group decreased and the proliferation slowed down. The G1 phase of SKOV3 cell cycle was inhibited and the apoptosis rate was increased （P〈0.05）. Conclusion： BTF3 can promote the growth and proliferation of ovarian cancer cell line SKOV3, and the expression of BTF3 gene in ovarian cancer may be closely related to the occurrence and development of ovarian cancer.