草莓果实中FaSAMDC基因的克隆、生物信息学及表达分析

Cloning,bioinformatics and expression analysis of FaSAMDC gene in strawberry fruits

【目的】为明确S-腺苷甲硫氨酸脱羧酶(SAMDC)在非呼吸跃变型草莓果实成熟中的作用。【方法】以‘北农香’草莓果实为试验材料,首先通过RT-PCR克隆FaSAMDC基因,其次通过SqRT-PCR分析发育果实(小绿、大绿、褪绿、白果、片红、全红)中的FaSAMDC基因的表达量。【结果】结果表明,FaSAMDC基因含有1 116bp开放阅读框,编码371个氨基酸,含有典型的脱羧酶保守功能结构域。随着草莓的果实发育,FaSAMDC表达量呈明显下降趋势并且在白果时期达到最低值,此后随着果实着色,表达量迅速上升并且成熟期达到最高。【结论】以上研究结果证实,S-腺苷甲硫氨酸脱羧酶可能参与草莓果实的成熟调控。

[Objective] To clarify the role of S-adenosylmethionine decarboxylase （SAMDC） in fruit ripening of non-climacteric strawberry .[Methods]The fruit of ＇Beinongxiang＂ strawberry fruit was used as material, the FaSAMDC gene was cloned by RT-PCR, and the mRNA expression levels of FaSAMDC gene in developmental fruits were studied by SqRT-PCR including the stages [small green （SG）, big green （BG）, de-greening （DG）, white （Wt）, partial red （PR）, full red （FR）] .[Results] The results showed that, FaSAMDC gene contains an open reading frame of 1 116 bp, encoding 371 amino acids, typically containing decarboxylase conserved functional domains.During the fruit development of strawberry, the expression of FaSAM- DC showed a decreasing trend and reached the lowest value in the white period; then, with the fruit coloring, the expression level increased rapidly and reached the highest level at full red stage.[Conclusion]These results suggested that S-adenosylmethi- onine decarboxylase may be involved in the regulation of strawberry fruit ripening.