纳米银对多菌种生物膜中粪肠球菌的杀菌作用

Bactericidal effect of nano-silver against E. faecalis growing in multi-species biofilm

目的:研究纳米银对多菌种生物膜中粪肠球菌的杀菌作用。方法:用粪肠球菌、产黑色素普雷沃菌以及具核梭杆菌构建多菌种生物膜85个。分别用0.1%(12~15 nm、100 nm)纳米银悬浊液以及2%的次氯酸钠溶液处理,然后通过qPCR方法检测样本中粪肠球菌的活菌量和粪肠球菌的细菌总量。配对t检验比较各组细菌样本临界循环数(Ct)。结果:各个实验组使用和不使用PMA之间粪肠球菌的细菌计数有着差别(P=0.000);将3个实验组使用和不使用PMA q PCR检测到的Ct值的差值进行比较:纳米银(12~15 nm)实验组最大,纳米银(100 nm)实验组次之,次氯酸钠实验组最小(P<0.05)。结论:纳米银对于多菌种生物膜中的粪肠球菌有着较强的杀菌作用,直径较小的纳米银杀菌作用较强。

Objective：To evaluate the bactericidal effects of nano-silver against Enterococcus faecalis（E.faecalis） growing in multi-species biofilm.Methods：85 biofilms were established using MBECTM P＆amp;G Assay with E.faecalis together with Fusobacterium nucleatum and P.melaninogenica.Thereafter,10 specimens were used as the controls,75 were randomly divided into 3 groups（n=25）,and treated with 0.1% nano-silver （12-15 nm） solution,0.1% nano-silver （100 nm） solution and 2% hypochlorite solution,respectively.Each sample was then separated into 2 different tubes.PMA was added to one of the tubes,and the other was left untreated.Then,DNA extraction and qPCR were performed.The cycle threshold（Ct） values between samples were compared by paired t test.Results：The Ct values of the samples treated with PMA were higher than that without PMA（P=0.000） in the group of 0.1% nano-silver solution（12-15 nm）was higher than that in the group of 0.1% nano-silver solution（100 nm）and 2% sodium hypochlorite solution.（P〈0.05）;the value in the group of 0.1% nano-silver solution（100 nm）was larger than that in 2% sodium hypochlorite solution（P〈0.05）.Conclusion：0.1% nano silver solution might have a strong bactericidal effect against against E.faecalis growing in multi-species biofilm.The bactericidal effect may be enhanced with the small size of silver particles.