羊蹄根对免疫性血小板减少性紫癜模型小鼠转化生长因子-β_1及其受体的影响

Effects of Rumex Japonicus Houttextract on Transforming Growth Factor-β_1 and Receptors in Thrombocytopenic Purpura Mice

目的:通过建立免疫性血小板减少性紫癜模型(ITP)小鼠,观察羊蹄根提取物对小鼠外周血转化生长因子(TGF-β_1)以及受体的影响,对羊蹄根提取物的免疫调节机制进行探讨。方法:将40只BALB/C小鼠随机分为4组,分别是阳性对照组、模型组、羊蹄根提取物组、阴性对照组。除阴性对照组外,其余各组均采用腹腔注射豚鼠抗小鼠血小板血清(APS)的免疫造模方法进行ITP造模。采用实时PCR方法及Western Blot法检测给药前与给药14d后各组小鼠的TGF-β_1、受体TGF-βR表达量的变化。结果:与阴性对照组比较,模型组TGF-β_1的表达量明显升高(P<0.05);TGF-β_1mRNA以及TGF-β_1蛋白表达量明显降低(P<0.05)。与模型组比较,羊蹄根提取物组TGF-β_1的表达量明显偏低(P<0.05);TGF-β_1mRNA以及TGF-β_1蛋白表达量明显偏高(P<0.05)。结论:羊蹄根提取物对ITP造模小鼠免疫功能具有调节作用,具体通过影响TGF-β_1以及受体实现。

Objective：To observe the effects of Rumex japonicus Houtt extract on transforming growth factor-β1 and its receptors in thrombocytopenic purpura mice model. Methods： 40 BALB/C mice were ran-domly divided into positive control group, model group, rumex japonicus houtt extract group and negative con-trol group. All ITP mouse model were made by injected guinea pig-antimouse platelet serum （GP-APS） except negative control group. The expressions of TGF-β1 and their receptors TGF-βR in the peripheral blood of mice were detected by the real-time PCR and Western Blot, and differences of their expression levels were analyzed. Results： The expression of TGF-β1 in ITP model group was significantly higher than that in the nega- tive control group （P〈0.05}, while the TGF-β mRNA and TGF-β protein expression in ITP model group were significantly lower than that in the negative control group （ P〈0.05 ）. The expression of TGF-β1 in rnmex japonicus houtt extract group was significantly lower than that in the ITP model group （ P〈0.05 ） , while the TGF-β mRNA and TGF-β protein expression in rumex japonicus houtt extract group were significantly higher than that in the ITP model group （P〈0.05）. Conclusion： Rumex japonicus houtt extract can regulate the immune function of ITP by in fluencing the expression of transforming growth factor-β, and its receptors.