两种不同慢传输便秘大鼠模型构建方法比较及对水通道蛋白的影响

Comparison of Two Kinds of Rat Model for Slow Transit Constipation and Expression of Aquaporins in Them

目的比较进食含复方地芬诺酯的饲料和用复方地芬诺酯混悬液灌胃复制大鼠慢传输便秘(STC)模型的差异。方法40只Sprague-Dawley大鼠随机均分为灌胃对照(A)组、灌胃(B)组、饲喂对照(C)组、饲喂(D)组。B组采用复方地芬诺酯混悬液灌胃14 d,A组用等量生理盐水灌胃,D组进食含有复方地芬诺酯的饲料120 d,C组进食普通饲料。造模成功后炭墨灌胃,观察各组肠道炭墨推进率;免疫组织化学方法检测水通道蛋白(AQP)3、AQP4蛋白含量。结果 A、B组各有2只大鼠死亡。肠道炭墨推进率D组低于B组(P<0.05);AQP3、AQP4蛋白水平D组均高于B组(P<0.05)。结论进食复方地芬诺酯饲料120 d较复方地芬诺酯灌胃14 d能够更安全、稳定地复制STC模型。AQP可能参与STC的发生、发展。

Objective To compare the rat model of slow transit constipation （STC） established with gavage or feeding compound diphenoxylate. Methods Forty Sprague-Dawley rats were randomly divided into gavage control group （A）, gavage group （B）, feeding control group （C） and feeding group （D） equally. Group B was given compound diphenoxylate suspension by gavage for 14 days while group A accepted isodose normal saline. Group D were given feed containing compound diphenoxylate for 120 days while group C accepted normal feed. Carbon ink was poured into stomachs to observe the propelling rate after modeling, and the content of aquaporin 3 and aquaporin 4 were detected with immunohistochemistry. Results There were two rats died in group A and group B, respectively. The carbon ink propelling rate was lower in group D than in group B （P〈0.05）. Both aquaporin 3 and aquaporin 4 were more in group D than in group B （P〈0.05）. Conclusion STC rat model can be established by eating diphenoxylate feed for 120 days, which is safer and more stable than that of gavage for 14 days. Aquaporins in colons may play a role in onset and development of STC.