参麦注射液对支气管哮喘患者外周血Thl7／Treg细胞的影响

Effect of Th17/Treg imbalance in the peripheral blood of asthma patients

目的探讨参麦注射液对支气管哮喘（简称哮喘）患者外周血Thl7／Treg细胞的影响及其意义。方法选取山西医科大学第一医院呼吸科急性发作期、缓解期哮喘患者各25例，选取山西医科大学第一医院体检中心肺功能正常、激发或者舒张试验阴性的健康人25例作为对照组，分别使用1640培养液及参麦注射液进行干预处理。抽取晨起空腹静脉血5ml，运用流式细胞仪对各组外周血Thl7细胞、Treg细胞分别占CD4^＋的比例及Thl7／Treg细胞的比值进行比较，同时检测血清中IL-10因子的水平。结果①各组问干预前CD4^＋Thl7细胞／CD4^＋T细胞、CD4^＋Thl7细胞／CD4^＋Treg细胞的比值，急性发作期组（3．05±1．27、1．49±1．78）高于缓解期组（2．38±0．93、0．61±0．24）及对照组（1．19±0．39、0．30±1．14），差异有统计学意义（Pd0．05），缓解期高于对照组，差异有统计学意义（P〈0．05）；CD4’Treg细胞／CD4’T细胞的比值，对照组（5．20±3．26）高于缓解期组（3．99±0．90）及急性发作期组（2．76±0．93），差异有统计学意义，（P〈0．05），缓解期组高于急性发作期组，差异有统计学意义（Pd0．05）。血清中IL-10因子的水平，对照组（5．28±1．31）高于缓解期组（4．46±0．83）及急性发作期组（3．90±0．64），差异有统计学意义（Pd0．05），缓解期组高于急性发作期组，差异有统计学意义（P〈0．05）。②同一组间CD4^＋Thl7细胞／CD4^＋T细胞、CD4^＋Thl7细胞／CD4^＋Treg细胞的比值，干预前高于干预后，差异有统计学意义（P〈0．05）。CD4^＋Treg细胞／CD4^＋T细胞的比值，干预后高于干预前，差异有统计学意义（P〈0．05）。血清中IL-10因子水平，干预后高于干预前，差异有统计学意义（P〈0．05）。结论哮喘患者外周血中存在Thl7／Treg细胞失衡，参麦注射液可以改善哮喘患者的Th17／Treg细胞失衡。

Objective To determine the value and effcet of ThlT/CD4＋ T cells and Treg/CD4＋ T cells in the peripheral blood of patients with asthma in acute exacerbation before and after the injection of shenmai intervention. Methods The study select 25 healthy people as the control group, 25 people with acute exacerbation of asthma and 25 people with non-acute exacerbation of asthma. These people were devided into 1640 intervention and the injection of shenmai intervention. Collecting fasting vein blood to determine the ratio of Thl7/CD4＋ T cells, Treg/CD4＋ T cells and the level of IL 10 by flow cytometry. Results @The level of CD4＋ Th17/CD4＋ T cells and Th17/CD4^＋ Treg cells： the acute exacerbation group （3.05±1.27,1.49±1.78） was higher than the remission group （2.38±0.93,0.61±0.24） and the control group （ 1.19±0.39,0.30±1.14）, the differences were statistically significant （ P 〈 0.05 ）, the remission group was higher than the control group, the differences were statistically significant （ P〈0.05）. The level of CD4^＋ Treg/CD4^＋ T cells： the the control group （5.20 ± 3.26） was higher than the remission group （3.99 ! 0.90） and the acute exacerbation group （2.76±0.93）, the differences were statistically significant （ P〈0.05）, the remission group was higher than the acute exacerbation group, the differences were statistically significant （ P % 0.05）. The level of IL-10： the the control group （5.28±1.31） was higher than the remission group （4.46 ± 0.83） and the acute exacerbation group （3.90±0.64）, the differences were statistically significant （ P %0.05）, the remission group was higher than the acute exacerbation group, the differences were statistically significant （ P %0.05）. QThe level of CD4^＋ Th17/CD4^＋ T cells and ThlT/CD4^＋ Treg cells： the pre-treatment was higher than the treated one, the differences were statistically significant （ P 〈 0.05）. The level of CD4^＋ Treg/CD4^＋ T cells：the treated one was higher than the pre-treatment, the differences were statistically significant （ P 〈0.05）. The level of IL-10 ：the treated one was higher than the pre-treatment, the differences were statistically significant （ P 〈0.05）.Conclusions The injection of shenmai can adjust this imbalance of ThlT/CD4＋ Treg cells that exist in the patients with asthma.