神经调节素对坐骨神经压迫性损伤的修复作用及其分子机制探究

Recovery effect of neuregulin on the sciatic nerve constriction injury as well as its molecular mechanism

目的:研究神经调节素(Nrg-1)对坐骨神经压迫损伤的修复作用及坐骨神经组织中Nrg-1下游信号分子的表达量。方法:选择SD大鼠作为实验动物并分为假手术+生理盐水干预组(A组)、坐骨神经压迫模型+生理盐水干预组(B组)、坐骨神经压迫模型+Nrg-1干预组(C组)。干预后2周、4周、8周时,测定坐骨神经功能参数;干预后8周时,测定坐骨神经组织中纤维化分子、Nrg-1下游分子的含量。结果:干预后2周、4周、8周时,B组大鼠坐骨神经的MCV、CMAP波幅显著低于A组,C组大鼠坐骨神经的MCV、CMAP波幅显著高于B组;干预后8周时,B组大鼠坐骨神经组织中CTGF、Col-Ⅰ、Col-Ⅲ、MMP2、MMP9、PTEN的含量显著高于A组,ErbB、Akt、PI3K的含量显著低于A组;C组大鼠坐骨神经组织中CTGF、Col-Ⅰ、Col-Ⅲ、MMP2、MMP9、PTEN的含量显著低于B组,ErbB、Akt、PI3K的含量显著高于B组。结论:神经调节素能够通过激活PI3K/Akt的方式发挥对坐骨神经压迫性损伤的修复作用。

Objective： To study the recovery effect of neuregulin （Nrg-1） on the sciatic nerve constriction injury as well as the expression of Nrg 1 downstream signaling molecules in sciatic nerve tissue. Methods： SD rats were selected as the experimental animals and divided into sham operation ＋ saline intervention group （group A）, sciatic nerve constrietion model ＋ saline intervention group （group B） and sciatic nerve constriction model ＋ Nrg-1 intervention group （group C）. 2 weeks, 4 weeks and 8 weeks after intervention, the sciatie nerve function parameters were deteeted; 8 weeks after intervention, the levels of fibrosis molecules and Nrg-1 downstream molecules in sciatic nerve tissue were determined. Results： Two weeks, 4 weeks and 8 weeks after intervention, sciatic nerve MCV and CMAP amplitudes of group B were significantly lower than those of group A, and the sciatic nerve MCV and CMAP amplitudes of group C were significantly higher than those of group B; 8 weeks after intervention, CTOF, Col-Ⅰ, Col-Ⅲ, MMP2, MMP9 and PTEN levels in sciatic nerve tissue of group B were significantly higher than those of group A while ErbB, Akt and PI3K levels were significantly lower than those of group A; CT- GF, Col-Ⅰ, Col Ⅲ, MMP2, MMP9 and PTEN levels in sciatic nerve tissue of group C were significantly lower than those of group ]3 while ErbB, Akt and PI3K levels were significantly higher than those of group B. Conclusions： Neuregulin can activate the PI3K/Akt to exert the recovery effect on sciatic nerve constriction injury.