非小细胞肺癌患者外周血NK细胞含量与肿瘤标志物含量、增殖分子表达量的相关性研究

Correlation of peripheral blood NK cell content with tumor marker content and proliferation molecule expression in patients with non-small cell lung cancer

目的:研究非小细胞肺癌(NSCLC)患者外周血NK细胞含量与肿瘤标志物含量、增殖分子表达量的相关性。方法:选择在我院确诊为NSCLC的80例患者作为研究的NSCLC组,同期在我院体检的96例健康志愿者作为研究的对照组。测定两组受试者外周血中NK细胞的含量以及血清中肿瘤标志物的含量,测定NSCLC组患者肺癌病灶和癌旁病灶组织中增殖分子的表达量。结果:NSCLC组患者外周血中NK细胞的含量均显著低于对照组;血清中CEA、CA125、Cyfra21-1、HE4的含量显著高于对照组且NK细胞高含量NSCLC患者血清中CEA、CA125、Cyfra21-1、HE4的含量均显著低于NK细胞低含量NSCLC患者;肺癌病灶组织中c-myc、c-FLIP、Livin、Moesin、k-ras的蛋白表达量均显著高于癌旁病灶组织且NK细胞高含量肺癌病灶组织中c-myc、c-FLIP、Livin、Moesin、k-ras的蛋白表达量均显著低于NK细胞低含量肺癌病灶组织。结论:NSCLC患者外周血中NK细胞含量显著减少且与癌细胞的增殖活力相关。

Objective： To study the correlation of peripheral blood NK cell content with tumor marker content and proliferation molecule expression in patients with non-small cell lung cancer （NSCLC）. Methods： A total of 80 patients who were diagnosed with NSCLC in our hospital between June 2014 and October 2016 were selected as the NSCLC group of the research, and 96 healthy volunteers who received physical examination in our hospital during the same period were selected as the control group of the research. The peripheral blood NK cell content and serum tumor marker contents of two groups of subjects were determined, and the proliferation molecule expression levels in lung cancer focus tissue and focus tissue adjacent to carcinoma of NSCLC group were determined. Results： Peripheral blood NK cell content of NSCLC group was significantly lower than that of control group; serum CEA, CA125, Cyfra21-1 and HE4 contents were significantly higher than those of control group, and serum CEA, CA125, Cyfra21-1 and HE4 contents of NSCLC patients with high NK cell content were significantly lower than those of NSCLC patients with low NK cell content; c-myc, c-FLIP, Livin, Moesin and k-ras protein expression in lung cancer focus tissue were significantly higher than those in focus tissue adjacent to carcinoma, and c-myc, c-FLIP, Livin, Moesin and k-ras protein expression in lung cancer focus tissue with high NK cell content were significantly lower than those in lung cancer focus tissue with low NK cell content. Conclusions： Peripheral blood NK cell content significantly reduces in patients with NSCLC and is associated with the cancer cell proliferation activity.