胃癌病变过程中恶性生物学分子表达与幽门螺杆菌感染的关系探究

Relationship between malignant biological molecule expression during the pathological process of gastric cancer and helicobacter pylori infection

目的:研究胃癌病变过程中恶性生物学分子表达与幽门螺杆菌(HP)感染的关系。方法:选择在我院接受手术切除的78例胃癌标本作为研究材料,收集胃癌组织及癌旁组织,采用快速尿素酶法试剂盒检测HP,采用荧光定量PCR试剂盒测定增殖、侵袭相关基因的mRNA表达量。结果:胃癌组织中EZH2、c-myc、PIK3CD、Vav3、UHRF1的mRNA表达量显著高于癌旁组织,Mst1、ING5、RASAL、Ecadherin的mRNA表达量显著低于癌旁组织;HP阳性胃癌组织中EZH2、c-myc、PIK3CD、Vav3、UHRF1的mRNA表达量显著高于HP阴性胃癌组织,Mst1、ING5、RASAL、E-cadherin的mRNA表达量显著低于HP阴性胃癌组织。结论:幽门螺杆菌对胃癌病变过程增殖、侵袭相关恶性生物学分子的表达具有调控作用。

Objective： To study the relationship between malignant biological molecule expression during the pathological process of gastric cancer and helicohacter pylori （HP） infection. Methods： A total of 78 cases of gastric cancer specimens that were surgically removed in our hospital between May 2013 and June 2015 were selected as the research material, the gastric cancer tissue and the tissue adiacent to carcinoma were collected, rapid urease test kits were used to test HP, and fluorescent quantitative PCR kits were used to determine the mRNA expression of proliferation- and invasion-related genes. Results= EZH2, c-mye, PIK3CD, Vav3 and UHRF1 mRNA expression in gastric cancer tissue were significantly higher than those in the tissue adjacent to carcinoma while Mstl, ING5, RASAL and E-cadherin mRNA expression were significantly lower than those in the tissue adjacent to carcinoma; EZH2, c-myc, PIK3CD, Vav3 and UHRF1 mRNA expression in HP-positive gastric cancer tissue were significantly higher than those in HP-negative gastric cancer tissue while Mstl, ING5, RASAL and E-cadherin mRNA expression were significantly lower than those in HP-negative gastric cancer tissue. Conclusions： Helicobaeter pylori have regulating effect on the expression of proliferation- and invasion-related malignant biological molecules during the pathological process of gastric cancer.