高强度聚焦超声联合介入栓塞化疗对晚期宫颈癌病灶生长及细胞侵袭的影响

Effects of high-intensity focused ultrasound combined with interventional chemoembolization on the advanced cervical cancer lesion growth and cell invasion

目的:研究高强度聚焦超声(HIFU)联合介入栓塞化疗对晚期宫颈癌病灶生长及细胞侵袭的影响。方法:选择本院收治的ⅡB-IVA期宫颈癌患者并随机分为两组,HIFU组接受HIFU联合介入栓塞化疗,对照组接受介入栓塞化疗。治疗前后测定血清中肿瘤标志物含量以及肿瘤病灶内抑癌基因、侵袭基因的表达量。结果:治疗后2、3、4周时,两组血清中TK-1、SCC、CA125的含量均低于治疗前且HIFU组患者治疗后2周时血清中TK-1、SCC、CA125的含量与对照组比较无差异,HIFU组患者治疗后3周、4周时血清中TK-1、SCC、CA125的含量显著低于对照组;治疗后4周时,两组肿瘤病灶内AIF、NDRG4、SARI、eIF4E3的mRNA表达量均高于治疗前,FAK、KGFR、MMP9的mRNA表达量均低于治疗前且HIFU组肿瘤病灶内AIF、NDRG4、SARI、eIF4E3的mRNA表达量显著高于对照组,FAK、KGFR、MMP9的mRNA表达量均低于对照组。结论:HIFU联合介入栓塞化疗能够较单纯介入栓塞化疗更为有效的抑制晚期宫颈癌病灶生长及细胞侵袭。

Objective： To study the effects of high-intensity focused ultrasound （HIFU） combined with interventional chemoembolization on the advanced cervical cancer lesion growth and cell invasion. Methods： Patients with stage IIB-IVA cervical cancer treated in Suining Hospital of TCM between May 2014 and October 2016 were selected and randomly divided into two groups, HIFU group received HIFU combined with interventional ehemoembolization, and the control group accepted interventional chemoembolization. The levels of tumor markers in serum as well as the expression of tumor suppressor genes and invasion genes in tumor lesions were determined before and after treatment. Results： 2 weeks, 3 weeks and 4 weeks after treat- ment, serum TK-1, SCC and CA125 levels of both groups were lower than those before treatment, serum TK-1, SCC and CA125 levels of HIFU group 2 weeks after treatment were not different from those of control group, and serum TK-1, SCC and CA125 levels of HIFU group 3 weeks and 4 weeks after treatment were significantly lower than those of control group; 4 weeks after treatment, AIF, NDRG4, SARI and elF4E3 mRNA expression in tumor lesions of both groups were higher than those before treatment while FAK, KGFR and MMP9 mRNA expression were lower than those before treatment, and .＆IF, NDRG4, SARI and eIF4E3 mRNA expression in tumor lesions ot＂ HIFU group were higher than those of control group while FAK, KGFR and MMP9 mRNA expression were lower than those of control group. Conclusion： HIFU combined with interventional chemoembolization can be more effective in suppressing the advanced cervical cancer lesion growth and cell invasion than interventional chemoembolization alone.