摘要
为推进鱼类物种鉴定,填补黑线鳕鱼核酸标准样品国内空白,本研究制备了黑线鳕鱼核酸标准样品。研究采用8家单位合作定值的方法,确定了该标准品的DNA质量为(2.02±0.07)μg,k=2;DNA纯度为(1.82±0.08),k=2。并对该标准品的均匀性、稳定性进行了测试,结果表明该样品均匀性与稳定性均达到国家标准样品的技术要求,可用于黑线鳕鱼物种鉴定中方法验证和质量控制。
To help identify marine fish and provide a reference standard for identification of Melanogrammus aeglefinus, a standard sample for Melanogrammus aeglefinus is established in this study. With collaboration of eight labs, concentration and quality of DNA are tested. The concentration value is (2.02-+0.07) txg (k=2) and the purity value of DNA is 1.82-+0.08 (k=2) which indicates good quality of samples. PCR and electrophoresis are used to test the uniformity, stability and value analysis of the samples. The results show that these samples in this study meet the technical requirements of national standards and that the method can be used for calibration and quality control of Melanogrammus aeglefinus.
出处
《科技导报》
CAS
CSCD
北大核心
2017年第9期100-106,共7页
Science & Technology Review
基金
质检公益性行业科研专项(2013IK291)
教育部新世纪优秀人才项目(NCET-11-0842)
统筹推进一流大学和一流学科建设经费(10301-0150200604)
中央民族大学学术团队建设项目(2015MDTD25C&2015MDTD13C)
关键词
黑线鳕鱼
物种鉴定
DNA标准样品
均匀性
稳定性
不确定度
Melanogrammus aeglefinus
molecular identification
standard sample
homogeneity
stability
uncertainty