PM2.5对支气管上皮细胞中VEGF、ROS的影响

Effect of PM2.5 on VEGF and ROS in bronchial epithelial cells

目的探讨细颗粒物(PM2.5)对支气管上皮细胞中血管内皮生长因子(VEGF)、活性氧类(ROS)的影响。方法培养支气管上皮细胞16HBE,分为4组:生理盐水对照组、25μg/ml PM2.5组、50μg/ml PM2.5组和100μg/ml PM2.5组,测定各组细胞增殖活性、ROS及VEGF表达水平。结果 50μg/ml PM2.5组和100μg/ml PM2.5组细胞增殖活性分别为(89.80±5.85)%和(77.20±7.70)%,低于生理盐水对照组(P<0.05);50μg/ml PM2.5组和100μg/ml PM2.5组染毒12和24 h ROS均高于生理盐水对照组(P<0.05),其中100μg/ml PM2.5组染毒12和24 h ROS水平均最高,分别为(16.60±0.40)和(24.01±0.55);50μg/ml PM2.5组和100μg/ml PM2.5组VEGF蛋白表达高于生理盐水对照组(P<0.05),其中100μg/ml PM2.5组VEGF蛋白表达水平最高(0.892±0.028)。结论 PM2.5能对支气管上皮细胞造成氧化损伤,上调VEGF蛋白表达。

Objective To investigate the effect of fine particulate matter （PM2.5） on vascular endothelial growth factor （VEGF） and reactive oxygen species （ROS） in bronchial epithelial cells. Methods Bronchial epithelial cells 16HBE were cultured and divided into saline control group, 25 μg/ml PM2.5 group, 50 μg/ml PM2.5 group and 100 μg/ml PM2.5 group. Cell proliferation activity and expressions of ROS and VEGF were measured in each group. Results In the 50 μg/ml PM2.5 group and the 100 μg/ml PM2.5 group, cell proliferation activity was （89.80 ± 5.85）% and （77.20 ± 7.70）% respectively, which was significantly lower than that in the saline control group （P 〈 0.05）; ROS level was higher than that in the saline control group after exposure for 12 and 24 h （P 〈 0.05）, among which the 100 μg/ml PM2.5 group had the highest ROS level after 12-h and 24-h exposure [（16.60 ± 0.40） and （24,01 ± 0.55） respectively]. In the 50 μg/ml PM2.5 group and the 100 μg/ml PM2.5 group, VEGF protein expression was higher than that of the saline control group （P 〈 0.05）, among which VEGF protein expression level in the 100 μg/ml PM2.5 group was the highest （0.892 ± 0.028）, Conclusions PM2.5 can cause oxidative damage to bronchial epithelial cells and up-regulate the expression of VEGF protein.