MicroRNA-101对上皮性卵巢癌耐药细胞SKOV3/DDP顺铂敏感性的影响

Effect of mi RNA-101 on chemosensitivity of SKOV3/DDP cells to Cisplatin

目的探讨MicroRNA-101(miRNA-101)对上皮性卵巢癌耐药细胞SKOV3/DDP顺铂敏感性的影响及相关作用机制。方法本研究通过细胞转染技术上调SKOV3/DDP细胞内miRNA-101的表达,采用RT-PCR检测miRNA-101及BDNF m RNA的表达情况;CCK-8检测SKOV3/DDP细胞顺铂敏感性及增殖力;Annexin V-FITC/PI流式细胞实验检测细胞凋亡率。结果与阴性对照组比较,miRNA-101转染组细胞miRNA-101表达量及细胞凋亡率增加[(1.000±0.022)vs(5.380±0.246),P<0.05],[(11.020±0.685)%vs(26.158±1.278)%,P<0.05];BDNF m RNA表达量降低[(1.000±0.042)vs(0.389±0.055),P<0.05];顺铂IC50及细胞增殖力均下降[(57.276±1.717)vs(33.176±2.465),P<0.05],[(1.776±0.030)vs(1.642±0.252),P<0.05]。结论 miRNA-101可有效增加SKOV3/DDP细胞对顺铂的药物敏感性,促进细胞凋亡。

Objective To explore the effect of miRNA-IO1 on Cisplatin sensitivity of chemo-resistant epithelial ovarian cancer SKOV3/DDP cells and the related mechanism. Methods In this study, the expression of miRNA-101 in SKOV3/DDP ceils was up-regulated by transfeetion. Meanwhile, RT-PCR assay was applied to detect the expressions of miRNA-IO1 mRNA and BDNF mRNA, and CCK-8 kit was used to measure the Cisplatin sensitivity and proliferative ability of SKOV3/DDP cells. In addition, the apoptosis of SKOV3/DDP cells was detected by Annexin V-FITC/PI flow cytometry. Results Compared with the control group, the expression of miRNA-101 and the apoptosis rate of miRNA-101 in the transfected group increased significantly [（1.000 ± 0.022） vs （5.380 ± 0.246）, P 〈 0.05; （11.020 ± 0.685） % vs （26.158 ± 1.278） %, P 〈 0.05]. The expression of BDNF mRNA decreased significantly [（1.000 ± 0.042） vs （0.389 ± 0.055）, P 〈 0.05], and the Cisplatin IC50 and the proliferative ability of SKOV3/DDP cells were obviously decreased after transfection [（57.276 ± 1.717） vs （33.176 ± 2.465）, P 〈 0.05; （1.776 ± 0.030） vs （1.642 ± 0.252）, P 〈 0.05]. Conclusions miRNA-101 could effectively enhance the Cisplatin sensitivity and promote the apoptosis of SKOV3/DDP cells.