百香果的组织培养研究

Passion fruit seed aseptic germination and plantlet regeneration

以百香果种子为外植体,先经75%酒精消毒30s,后用无菌水清洗,再采用0.1%Hg Cl2以不同灭菌时间(6min、8min、10min)进行灭菌,以MS为基本培养基进行培养。将萌发出来的无菌植株按叶、茎、根材料,分别接到愈伤组织的培养基上进行诱导。同时对百香果无菌芽进行诱导丛生芽和生根实验。结果表明:采用0.1%Hg Cl2处理外植体8min,百香果种子的污染率较低并且萌发率较高;最佳诱导愈伤组织培养基为MS+2-4D 2 mg/L+GA3 0.2 mg/L+蔗糖20g/L+琼脂5.3g/L,p H6.8;最佳诱导丛生芽培养基为MS+6-BA2mg/L+NAA0.2mg/L+蔗糖20g/L+200g/L土豆泥+琼脂粉5.3g/L,p H6.8;最佳诱导生根培养基为MS+IBA2mg/L+NAA 0.2mg/L+活性炭2g/L+蔗糖20g/L+琼脂粉4.5g/L,p H6.8。

With passion fruit seed as explant,first by 75% alcohol disinfection 30s,with sterile water after cleaning,use 0.1% HgCl2 in different sterilization time （6min,8min,10min） for sterilization,to MS as the basic culture medium for culture,joined with different hormones into culture medium for culture get sterile passion fruit plant material.To grow out of the sterile plants cut into leaf, stem,root,such as material,culture medium on the callus induction.And induction of multiple shoot clumps in the passion fruit plants and roots of experiment.The results showed that the 8min 0.1% HgC12 used as explant,passion fruit seed pollution rate and low growth rate is higher;Best callus induction medium for MS ＋ 2-4 d 2mg/L ＋ GA such three types of 0.2mg/L ＋ sugar 20g/L ＋ AGAR powder 5.3g/L,pH6.8;Best for induction of multiple shoot clumps medium MS ＋ 6-BA2mg/L ＋ NAA0.2mg/L ＋ sugar 20g/L ＋ 200g/L mashed potatoes ＋ AGAR powder 5.3g/L,pH6.8;Best rooting medium for MS ＋ IBA ＋ NAA 2mg/L,0.2mg/L ＋ powdered carbon 2g/L ＋ sugar 20g/L ＋ AGAR powder 4.5g/L,pH6.8.