基于高通量测序患炭疽病草莓根际与健康草莓根际细菌群落的比较研究

A Comparative Study on Rhizosphere Soil Bacterial Communities of Healthy Strawberry and Infected Strawberry with Anthracnose by High-throughput Sequencing

研究旨在比较患炭疽病草莓与健康草莓根际土壤细菌群落多样性,了解其细菌群落生态特征,为找到有效的生防菌奠定基础。本研究基于土壤细菌16S r DNA V3-V4区基因多样性,利用Illumina Miseq高通量测序技术进行比较研究。研究得出:与健康对照相比,患病土壤细菌的OTUs数目及多样性指数均较低;在门水平上,变形菌门、拟杆菌门、浮霉菌门等是2种生境共有的优势类群;在属水平上,2种生境的优势菌丰度有差异,患病土壤的假单胞菌属丰度显著降低,气单胞菌属丰度显著升高;而且,健康土壤的解毒类菌、固氮菌、光合菌和硝化菌丰度均高于患病土壤。健康草莓根际土壤细菌丰富度及多样性均高于患病草莓,2种生境细菌菌群结构有差异,且健康土壤中对植物有益的功能微生物群体含量高于患病土壤。

The objectives were to compare the diversity of rhizosphere soil bacterial communities of healthy strawberry and infected strawberry with anthracnose, study the bacterial community ecological features involved, and provide evidence for further biocontrol bacteria screening. The diversity analysis was based on the variation of 16S rDNA V3-V4 area and performed by the Illumina Miseq high-throughput sequencing platform. The results showed that the infection resulted in reduced number of operational taxonomic units （OTUs） and diversity index when compared with the healthy specimens. Proteobacteria, bacteroidetes and planctomycetes were dominant shared by the two kind specimens at the phylum level. The two kind specimens had different dominant bacterial compositions at the genus level. In disease soil, the abundance of Pseudomonas was significantly decreased while the abundance of Aeromonas was significantly increased. In addition, the abundance of detoxification bacteria, nitrogen-fixing bacteria, photosynthetic bacteria and nitrifying bacteria in healthy soil were higher than those in disease soil. The rhizosphere soil bacteria abundance and the diversity of healthy strawberry were higher than those of infected strawberry. The structures of bacterial flora were quite different from each other in the two kind specimens. And the relative abundances of beneficial functional microbial groups in healthy soils were higher than that of the disease soils.