大黄素对哮喘小鼠肺组织形态及BALF中炎性细胞因子分泌水平的影响

Effects of Emodin on the Pulmonary Histology and the Inflammatory Cytokine Levels of BALF in Asthma Mice

目的:研究大黄素对哮喘小鼠肺部炎症及肺泡灌洗液(BALF)中炎性细胞因子分泌水平的影响,初探其作用机理。方法:采用卵清蛋白(Ovalbumin,OVA)致敏加激发法建立支气管哮喘小鼠模型。20只雌性C57BL/6小鼠随机分为正常组、模型组、大黄素组及阳性药对照组,实验结束后观察各组小鼠整体情况和肺组织病理学改变,收集肺泡灌洗液进行细胞因子检测。结果:模型组小鼠可见呼吸急促、喘息、打喷嚏、抓耳挠鼻、弓背、大小便失禁等症状,BALF中白介素(Interleukin,IL)-4、IL-5、IL-17A含量明显升高(P<0.01);肺组织病理学结果为支气管及血管周围大量炎性细胞浸润,肺泡结构不完整,伴支气管上皮损坏甚至纤维化等明显改变。经大黄素治疗后,哮喘小鼠上述症状、体征及病理改变均有所改善,且炎性细胞因子水平明显降低。结论:大黄素能够在一定程度上改善支气管哮喘小鼠肺部的症状及病理变化,抑制IL-4、IL-5、IL-17A的分泌。

Objective： To explore the effects of emodin on lung inflammation and inflammatory cytokine levels in BALF of mice with OVA-induced asthma. Methods ： The method of OVA sensitization and challenge was used to establish bronchial asthma mice models. 20 female C57BL/6 mice were randomly divided into normal group, model group, emodin group and positive con- trolled group. Each medication group was given relevant medicine for gavage. The overall situations as well as morphological changes from all groups were observed. The levels of cytokines in BALF of mice were detected. Results： Mice from the model group showed shortness of breath, wheezing, sneezing, scratching ear and nose, fecal incontinence etc. Compared with the con- trolled group, their pulmonary histological results showed a large number of inflammatory cells infiltrated around the bronchi and vessels, uncompletedalveolar structure associated with bronchial epithelial damage and even fibrosis change. What＇ s more, their levels of IL-4, IL-5, IL-17A in BALF significantly increased （P 〈 0.01 ） , compared with the controlled group. After treatment with emodin, the above symptoms, signs and pathologic changes from asthmatic mice were alleviated. Their inflammatory levels were also reduced. Conclusion： Emodin might alleviate pulmonary inflammation of bronchial asthma mice modelprobably through inhibiting the levels of IL-4, IL-5, IL-17A.