EGFRvⅢ与CXCR4在头颈部鳞状细胞癌组织中表达意义的初步研究

Preliminary study on the expression of EGFRvⅢ and CXCR4 in head and neck squamous cell carcinoma

目的:检测EGFRvⅢ和CXCR4在头颈部鳞状细胞癌(HNSCC)组织中的表达情况,初步探讨其在侵袭、转移中可能的作用。方法:选取HNSCC患者60例,其中喉鳞状细胞癌44例,下咽鳞状细胞癌14例,鼻腔鼻窦鳞状细胞癌2例;根据AJCC 2010pTNM分期,Ⅲ期17例,Ⅳa期40例,Ⅳb期3例;肿瘤分化程度:高分化13例,中分化31例,低分化16例;取肿瘤原发灶和(或)淋巴结转移灶组织作为实验组,取10例癌旁正常黏膜组织作为对照组。利用免疫组织化学法分别观察肿瘤的原发灶、转移灶及对照组中EGFRvⅢ和CXCR4的表达情况。利用Image-Pro Plus对免疫组织化学结果行半定量分析;利用SPSS 23.0和Graphpad-Prism 5.0对结果进行统计分析。结果:EGFRvⅢ和CXCR4在HNSCC均高表达,其表达与患者的年龄、性别、部位、分化程度、T分级、肿瘤分期均无明显相关性(P>0.05),但与N分级有明显相关性(P<0.05);病理证实淋巴结转移的癌组织中均有高表达;但原发灶和转移灶之间的表达差异无统计学意义;实验组与对照组之间差异明显(P<0.05);EGFRvⅢ和CXCR4无明显正相关(r=0.144,P>0.05)。结论:EGFRvⅢ和CXCR4均促进了晚期HNSCC的侵袭和转移,两者之间不具有正相关性,但可能有一定的协同作用。

Objective：The aim of this study is to detect the expression of EGFRvⅢ and CXCR4 in HNSCC and to explore its possible mechanism. Method..We selected 60 cases of HNSCC from May 2013 to November 2016 in our hospital for surgical treatment, including 44 cases of laryngeal squamous cell carcinoma, 14 cases of hypo- pharyngeal squamous cell carcinoma, and 2 cases of nasal squamous cell carcinoma, pTNM staging according to AJCC 2010,Ⅲ stage 17 cases, Ⅳa stage 40 cases, and Ⅳb stage 3 cases; according to tumor differentiation de- gree： 13 cases of high differentiation, moderately differentiation in 31 cases, and 16 cases of low differentiated; primary tumor, positive lymph node metastasis as the experimental group, and 10 cases of adjacent tissues are taken as control（control group）. The expression of EGFRvⅢ and CXCR4 in primary tumor, metastasis and con- trol group was observed by immunohistochemistry. The immunohistochemieal results are semi quantitatively ana- lyzed by Image-Pro Plus. All analyses were conducted using the SPSS 23.0 and Graphpad-Prism 5.0. Result： The expression of EGFRvⅢ and CXCR4 in HNSCC is higher than control, which was not correlated with age, gender, location, degree of differentiation, T grading, and TNM stage（P〉0.05）. But there was a significant correlation with N grading（P〈0.05）. The pathology result shows lymph node metastasis in cancer tissues were high expres- sion, but the difference in expression between primary and metastatic tumors are not statistically significant. There is no significant positive correlation between EGFRv Ⅲ and CXCR4 （r= 0.144, P〉 0.05）. Conclusion： Both EGFRvⅢand CXCR4 promote the invasion and metastasis of advanced HNSCC, however, there may be some synergistic effect between them.