膳食5'-核苷酸对大鼠酒精性肾损伤保护作用的研究

Protective effect of dietary 5'-nucleotides on alcohol-induced kidney injury of mice

为研究膳食5'-核苷酸对大鼠酒精性肾损伤的保护作用,选取35只SPF级雄性Wistar大鼠,随机分成5组:普通对照组、酒精组、葡萄糖等热量组、酒精+0.04%核苷酸干预组、酒精+0.16%核苷酸干预组。连续灌胃8周后,测定体重、肾体比、血肌酐(SCr)、尿肌酐(UCr)、血尿素氮(BUN)及24 h尿蛋白等相关指标;计算内生肌酐清除率(Ccr);检测血清炎症因子细胞间黏附分子-1(ICAM-1)、单核细胞趋化因子-1(MCP-1)水平;HE染色观察肾脏组织病理学改变;免疫组化法观察肾脏足细胞标志蛋白PCX-1、肾间质转化生长因子-β(TGF-β)和纤连蛋白(FN)的表达。结果表明:膳食5'-核苷酸能够降低酒精引起的大鼠24 h尿蛋白、血肌酐升高并提高内生肌酐清除率(P<0.05),0.16%核苷酸干预组还能显著降低酒精引起的大鼠血尿素氮升高(P<0.05);减轻酒精导致的肾脏组织结构病理改变及血清炎症因子ICAM-1、MCP-1水平的升高(P<0.05);同时减轻甚至逆转酒精导致的肾脏PCX-1、TGF-β和FN表达的改变。由此可知,膳食5'-核苷酸可以通过减轻血液炎症反应,调节肾脏蛋白质分子的表达,对酒精造成的大鼠肾损伤产生一定的保护作用。

This paper investigates the protective effects of dietary 5′-nucleotides on alcohol-induced kidney injury of mice. A total of 35 specific pathogen free （SPF） male Wistar mice are randomly divided into 5 groups given alcohol, equivalent distilled water or an isoealorie amount of dextrose intragastrieally for 8 weeks. The two groups of alcohol-induced rats are fed with AIN-93G rodent diets including 0.04% and 0.16% 5′-nucleotides, respectively. Body weight （bw）, renal function indexes and serum concentration of ICAM-1 and PCM-1 are measured, and renal pathologic changes stained by hematoxylin and eosin stain are observed by election microscope. The result shows that dietary 5′-nucleotides can reduce the rise of urinary protein per 24 h and level of blood serum ereatinine （SCr） induced by alcohol, as well as the blood urea nitrogen （BUN） in the 0.16% NTs group. And compared to the alcohol group, the endogenous ereatinine clearance are decreased significantly in the 0.04% and 0.16% NTs groups. The renal lesions present glomerular mesangium proliferation, inflammatory cell infiltration in tubule-interstitial and vaeuolation or swell of renal tubule epithelium in the alcohol group, which, are less in both NTs groups. The serum concentrations of ICAM- 1 and PCM- 1 of NTs groups are also lower than those of the alcohol group. What＇ s more, the expression changes of PCX-1, TGF-β and fibroneetin （FN） in kidney induced by alcohol are repaired by NTs supplement. In conclusion, the dietary 5′-nueleotides can protect kidney from injury induced by alcohol in mice.