七氟醚对基底前脑区抑制性中间神经元电活动的影响

Effect of sevoflurane on the electric activities of inhibitory interneurons in basal forebrain area

目的观察七氟醚对基底前脑区抑制性中间神经元电活动的影响,为吸入全麻药物的作用机制提供实验依据。方法 2~3周龄C57BL/6小鼠,切取脑片,运用全细胞膜片钳技术钳制基底前脑区神经元,灌流混合七氟醚的人工脑脊液,记录动作电位和抑制性突触后电流。结果七氟醚可使基底前脑区抑制性中间神经元自发放电频率增加(P<0.001),增加去极化电流引起的动作电位频率(P<0.05),并且增加锥体神经元抑制性突触后电流频率(sIPSC)(P<0.05),而动作电位依赖的抑制性突触后电流(mIPSC)没有明显变化。结论基底前脑区抑制性中间神经元参与了七氟醚的麻醉效应。

Objective To provide new experimental evidences associated with the mechanisms of inhaled anesthetics, the effects of sevoflurane on the electric activities of inhibitory interneurons in basal forebrain area （BF） were observed. Methods C57BL/6 mice, aged 2-3 weeks, were used and BF sections were cut for whole patch-clamp recording. Artificial cerebrospinal fluid containing sevoflu- rane was given and action potential, inhibitory postsynaptic potential were recorded. Results Sevoflu- rane could increase the frequency of firing rate of inhibitory interneurons in basal forebrain area （P〈 0. 001）, which could increase the frequency of action potential caused by depolarization current （P〈 0. 05）, and increase the frequency of spontaneous inhibitory postsynaptic currents of pyramidal neu- rons （P〈0. 05）, while AP-depended miniature inhibitory postsynaptic currents were not significantly changed. Conclusion The basal forebrain inhibitory interneurons are involved in the anesthetic effect of sevoflurane.