马铃薯块茎创伤木栓化过程中形态生理指标及内源激素的变化

Changes of morphological and physiological indices and endogenous hormones during potato tuber wound-healing suberization

本试验以马铃薯(Solanlum tuberosum)完整块茎为对照,马铃薯块茎纵切为创伤处理,主要研究马铃薯块茎创伤愈合过程中块茎内源激素、木质素合成关键酶(苯丙氨酸解氨酶、过氧化物酶)及创伤周皮形态解剖结构的变化。实验结果表明马铃薯块茎受创后苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)酶活明显增加,内源激素脱落酸(ABA)和玉米素核苷(ZR)含量明显增加,而赤霉酸(GA)含量降低。块茎创伤处理后创面在第4天形成木栓形成层,在第10天进入创伤周皮稳定增长的时期。同时,在处理块茎的未创伤处,其周皮厚度和层数均优于对照。实验结果表明马铃薯块茎创伤后由于ABA、ZR和GA等内源激素协同调控及POD、PAL酶活增加,促进了创伤周皮的形成,使受创的马铃薯块茎完成创伤木栓化。

In this study, we investigated the changes of endogenous hormones, the activities of relevant enzymes of lignin synthesis including phenylalanine ammonia-lyase （PAL） and peroxidase （POD）, and the anatomical structure of the wound periderm during potato （Solanlum tuberosum） wound-healing suberiza- tion. After wound treatment PAL and POD activities were significantly increased and the contents of ab- scisic acid （ABA） and zeatin riboside （ZR） were higher than untreated control tubers, but the content of gib- berellic acid （GA） was lower than untreated ones. During the day 3-4, phellogen formed on the hurt side of the tubers. The cell layers of peridem was 6 and the thickness and layers of periderm in the unhurt areas periderm increased steadily in day 10. Meanwhile the of treated tuber were higher than the control. The ex- perimental results show that the regulation of some endogenous hormones and the increase of PAL and POD activities promoted the wound-healing suberization.